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To get started with this blank TiddlyWiki, you'll need to modify the following tiddlers:
* SiteTitle & SiteSubtitle: The title and subtitle of the site, as shown above (after saving, they will also appear in the browser title bar)
* MainMenu: The menu (usually on the left)
* DefaultTiddlers: Contains the names of the tiddlers that you want to appear when the TiddlyWiki is opened
You'll also need to enter your username for signing your edits: <<option txtUserName>>
These InterfaceOptions for customising TiddlyWiki are saved in your browser

Your username for signing your edits. Write it as a WikiWord (eg JoeBloggs)

<<option txtUserName>>
<<option chkSaveBackups>> SaveBackups
<<option chkAutoSave>> AutoSave
<<option chkRegExpSearch>> RegExpSearch
<<option chkCaseSensitiveSearch>> CaseSensitiveSearch
<<option chkAnimate>> EnableAnimations

----
Also see [[AdvancedOptions]]
<<importTiddlers>>
! Introduction
The algorithm is inspired by the meristogram of Huffman & Bullock [Huffman DG & Bullock WL: ''Meristograms: graphical analysis of serial variation of proboscis hooks of //Echinorhynchus// (Acanthocephala)'' //Systematic Zoology// 1975, 24:333-345]. 

! Data
Hook length and base measurements are recorded from at least one longitudinal row per specimen (see [[How to Measure Hooks]]). To demonstrate the algorithm we have data from three specimens: 1-3.

[img[Profiles of three longitudinal rows of hooks.|images/3_hook_rows.png]]

! Standardization of hook positions
The number of hooks per longitudinal row is variable, both within and between specimens. In this example specimens 1 and 2 have the same number of hooks per row (9) and so each numbered hook (1:9) on one proboscis can be compared directly to the same numbered hook on the other proboscis. However, on proboscis 3 there are 11 hooks and therefore hooks 10 and 11 do not have an equivalent hook on the other two proboscides. Furthermore, hooks 1:9 on proboscis 3 are probably not from homologous regions of the proboscis to hooks 1:9 on proboscides 1  and 2. To resolve this issue we standardize the hook positions using Huffman & Bullock's method; the counted position number of each hook in a given row is multiplied by 100 and divided by n + 1, where n = the total number of hooks in the row and the constant 1 is a corrective factor for centering the data points in graphs. The figure below shows length measurements plotted against standardized hook position.

[img[Hook length plotted against standardized hook position.|images/hook_length_vs_std_pos.png]]

Homologous regions of the proboscis can now be compared across the three specimens. However, the '50% position' is the only site where individual hooks can be compared directly. If the data were entered into a matrix where rows were length measurements and columns were standardized hook positions, there would be many missing values. For example, at the 10, 20, 30, 40, 60, 70, 80 and 90 '% positions' there would be missing data for specimen 3. Such an incomplete data matrix is unsuitable for multivariate statistical analysis.

! Moving Average Routine
Huffman and Bullock used a moving average routine to smooth the curves in their meristogram. Here, their approach is adopted to generate variables (hook dimensions) which may be compared across all individuals in a collection of worms. The moving average routine is applied to the data from each row of hooks and considers a user-defined segment of the percent-position axis for each measurement (length and base). The minimum size (m) for the moving average segment is calculated as:
m=ceiling((100/h+1)+1)
where h is the smallest number of hooks per longitudinal row recorded for the collection of worms. In the current example, the lowest number of hooks per row is 9 and so the minimum segment size is 11. If the size of the moving average segment was set below this value, one of more segments would not contain any hooks and therefore missing values would be generated by the moving average routine.

The segment advances through the data from anterior to posterior in 1% increments. After each advance of the segment, the arithmetic mean for the length measurements and base measurements of all hooks which fall within the segment are calculated. Note that the moving average routine is applied to each row of hooks, generating a length profile and a base profile for each row. This is different to Huffman & Bullock's meristogram, where data from all worms in a collection are summarized, to generate a single pattern describing the entire collection. Hook length profiles (generated using a moving average segment size of 11%) for the three example hook rows are shown in the figure below.
[img[Proboscis profiles for 3 specimens.|images/3profiles.png]]


! Pattern Recognition
The next step in the algorithm is unsupervised pattern recognition, which attempts to discover natural groups of hook rows sharing similar length and base profiles. The first stage of the pattern recognition process is to perform a principal component analysis (PCA) on the log transformed data (both length and base measurements).

Pattern recognition aims to identify morphotypes, //i.e.// groups of worms with similar proboscis profiles.
!!! Principal Component Analysis
The profiles generated from the moving average routine form a high-dimensional data set of almost 200 observations per hook row. Principal component analysis (PCA) is used to transform this large number of usually correlated variables into a smaller number of uncorrelated variables called principal components (PCs). If the PCA is effective, most of the variation in the dataset will be described by the first two PCs. Plotting the scores for the first two PCs will then show similarities between individual hook rows (see figure below), while the PC loadings (not shown) indicate the relative importance of the original variables.

[img[Proboscis profiles for 3 specimens.|images/pca3.png]]
In the above plot, PC1 (the PC describing the largest amount of variation in the data) separates specimen 1 from the other 2 specimens.

!!! Cluster Analysis
Hierarchical cluster analysis (complete agglomeration method with Euclidean distances) is performed on the first two principal components (//i.e.// the PCs describing the majority of the variation in the data) to provide an alternative visualization of morphological similarity between specimens.
[img[Cluster dendrogram for 3 specimens.|images/dendrogram3.png]]
!R classes and functions
The script [[acanthocephalan_proboscis_profiler.R | downloads/acanthocephalan_proboscis_profiler.R]] provides a number of classes and functions, for more detail see:

[[R class]]

[[R function]]
!1 Preparation
If R isn't already installed on your machine, you can download it for free from the [[R project website|http://www.r-project.org]].

Running Acanthocephalan Proboscis Profiler from the CLI will be easy if you already have some experience with the R environment. Don't worry if you don't have any experience with R, you should still be able to follow this guide. However, if you are interested in learning more about R you will find many introductory tutorials [[here|http://cran.r-project.org/other-docs.html]]. Official manuals can be found [[here|http://cran.r-project.org/manuals.html]].

N.B. If you are unfamiliar with R and have access to a computer running Windows XP/Vista, you may find it easier to use the [[Graphical User Interface (GUI)|Graphical User Interface]].

!2 Start R

!!!Linux/Unix
At the command line type ''R''

//{{{
mw283@ubuntu:~$ R

R version 2.9.2 (2009-08-24)
Copyright (C) 2009 The R Foundation for Statistical Computing
ISBN 3-900051-07-0

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

  Natural language support but running in an English locale

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> 

//}}}

!!!Mac OSX
Go to applications and double-click on the R icon. Alternatively, open a terminal window and type ''R''

[img[Mac OSX R Console|images/macosxRconsole.png]]

!!!Windows
''Start Menu'' -> ''All Programs'' -> ''R''

[img[Windows R Console|images/windowsRconsole.jpg]]

!3 Read in source code
The latest version of the program can be sourced directly from our website (this will ensure you get the most up-to-date version):
//{{{
> source("http://acanthocephala.sourceforge.net/downloads/acanthocephalan_proboscis_profiler.R")
//}}}

!4 Read in raw data
Use [[readHookMeasurements]] function to read in your own data file (see [[Input File Format]]) or one of our example data files:
//{{{
> echRaw <- readHookMeasurements("http://acanthocephala.sourceforge.net/downloads/Echinorhynchus_spp.csv", notes="Data from three Echinorhynchus species")
//}}}
This creates an object of class hookMeasurements. To get a summary of the raw data use:
//{{{
> summary(echRaw)
	Hook Measurements Summary

Number of groups: 3
Total number of specimens: 54

Number of specimens per group:

       group number of specimens
1  E. gadi A                   4
2  E. gadi B                   4
3 E. truttae                  46

Notes:
Data from three Echinorhynchus species

//}}}

!5 Generate proboscis profiles
Use [[profileProboscides]] function:
//{{{
> echProfiles <- profileProboscides(echRaw)
//}}}

Using default settings, profileProboscides generates profiles using the smallest moving average interval that can be applied to the raw data. To find out what moving average interval was applied, use the [[movAvgSeg]] function:
//{{{
> movAvgSeg(echProfiles)
[1] 11
//}}}

For a summary of the profiles use:
//{{{
> summary(echProfiles)
	Proboscis Profiles Summary

Moving Average Segment: 11
Number of groups: 3
Total number of specimens: 54

Number of specimens per group:

       group number of specimens
1  E. gadi A                   4
2  E. gadi B                   4
3 E. truttae                  46

Notes:
Data from three Echinorhynchus species

//}}}

!6 Principal Component Analysis
Perform principal component analysis (PCA) on proboscis profiles:
//{{{
> echPCA <- prcomp(allHookMetrics(echProfiles))
//}}}
echPCA is an object of class ''prcomp''

To see the amount of variation described by the first two principal components use:
//{{{
> summary(echPCA)$importance[1:3,1:2]
                            PC1       PC2
Standard deviation     1.517088 0.6849417
Proportion of Variance 0.668140 0.1361900
Cumulative Proportion  0.668140 0.8043300
//}}}

Plot the scores for the first two principal components using:
//{{{
> plot(echPCA$x)
//}}}
[img[PCA scores plot|images/cli_pca.png]]

To use a distinct colour and symbol for each group, use:
//{{{
> plotColours <- gsub("E. truttae", "red", (gsub("E. gadi B", "green", (gsub("E. gadi A", "blue",annotation(echProfiles)$group)))))
> plotSymbols <- as.numeric(gsub("E. truttae", 3, (gsub("E. gadi B", 2, (gsub("E. gadi A", 1,annotation(echProfiles)$group))))))
> plot(echPCA$x, col=plotColours, pch=plotSymbols)
> legend("bottomleft", as.character(unique(annotation(echProfiles)$group)), pch=c(1,2,3), col=c("blue", "green", "red")) 
//}}}
[img[PCA scores plot - groups labeled|images/cli_pca2.png]]

Identify individual points (specimens) in the plot by running the following command and then left-clicking on the points in the plot:
//{{{
> identify(echPCA$x, labels=as.character(annotation(echProfiles)$specimen))
//}}}
[img[PCA scores plot - individual specimens identified|images/cli_pca3.png]]
Right-click on graphics device to end indentification.

!7 Cluster Analysis
Perform cluster analysis and display the result as a dendrogram:
//{{{
> dendrogram <- hclust(dist(echPCA$x[,1:2], method='euclidean'), method='complete')
> plot(dendrogram, labels = paste(annotation(echProfiles)$group, annotation(echProfiles)$specimen, sep=' - '), xlab='', sub='', ylab='Distance')
//}}}
[img[Cluster Dendrogram|images/cluster_dendrogram.png]]

!8 Plot Profiles
First use the [[proboscisProfilesDataFrame]] function to create a data frame of your proboscis profiles data:
//{{{
> echDF <- proboscisProfilesDataFrame(echProfiles)
//}}}

Plots of length and base profiles can then be created using:
//{{{
> plotColours <- gsub("E. truttae", "red", (gsub("E. gadi B", "green", (gsub("E. gadi A", "blue",echDF$group)))))
> plotSymbols <- as.numeric(gsub("E. truttae", 3, (gsub("E. gadi B", 2, (gsub("E. gadi A", 1,echDF$group))))))
> par(mfrow=c(2,1))
> plot(echDF$position, echDF$length, pch=plotSymbols, col=plotColours, ylim=c(2.9,4.5), main="Length Profiles", xlab="Position (%)", ylab=expression(paste('Log Length', ' ', '(',mu,m,')')))
> legend("bottom", as.character(unique(echDF$group)), pch=c(1,2,3), col=c("blue", "green", "red"))
> plot(echDF$position, echDF$base, pch=plotSymbols, col=plotColours, main="Base Profiles", xlab="Position (%)", ylab=expression(paste('Log Base', ' ', '(',mu,m,')')))
> legend("bottom", as.character(unique(echDF$group)), pch=c(1,2,3), col=c("blue", "green", "red"))
//}}}
[img[Length and Base Profile Plots|images/cli_profile_plots.png]]

!9 Write Profile Data to File
Proboscis profile data can be written to a comma-separated-value file which can then be read into other applications.

To export both length and base profiles use the [[writeAllHookMetrics]] function:
//{{{
> writeAllHookMetrics(echProfiles, "Echinorhynchus_spp_profiles.csv")
//}}}

To export length profiles only use the [[writeHookLengthProfiles]] function:
//{{{
> writeHookLengthProfiles(echProfiles, "Echinorhynchus_spp_length_profiles.csv")
//}}}

To export base profiles only use the [[writeHookBaseProfiles]] function:
//{{{
> writeHookBaseProfiles(echProfiles, "Echinorhynchus_spp_base_profiles.csv")
//}}}
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Acanthocephalan Proboscis Profiler is developed by Matt Wayland. 

E-mail: mwayland[at]users.sourceforge.net

The ''Sourceforge'' project page for this software can be found [[here|https://sourceforge.net/projects/acanthocephala/]].
[[Introduction]]
!Collecting data
[[How to Measure Hooks]]
[[Input File Format]]

!Algorithm
For a graphical description of the proboscis profiler method, see [[Algorithm]]

!Graphical User Interface (GUI)
[[GUI Installation]]
[[GUI Quick Start Guide]]
[[GUI Advanced Options]]

!Command Line Interface (CLI)
[[CLI Quick Start Guide]]
[[CLI Advanced Use]]

!Publication
[[Wayland MT. Proboscis profiler: a tool for detecting acanthocephalan morphotypes. Systematic Parasitology 2010 Jul;76(3):159-67. | http://link.springer.com/article/10.1007/s11230-010-9245-z]]

Reprints available on request (e-mail: mwayland[at]users.sourceforge.net).
!R source code
The [[R source code|downloads/acanthocephalan_proboscis_profiler.R]] is platform independent. The source code is used from the [[command line interface (CLI) |CLI Quick Start Guide]] of [[R|http://www.r-project.org/]]. If you don't already have R installed on your computer it can be downloaded from the [[R-project website|http://www.r-project.org/]].

[[Download R source code for Acanthocephalan Proboscis Profiler|downloads/acanthocephalan_proboscis_profiler.R]]

!Windows GUI (Graphical User Interface)
Please read the [[installation instructions |GUI Installation]] before launching the installer.
[[Install Acanthocephalan Proboscis Profiler GUI|install/setup.exe]]

Source code for the windows GUI is also available in the form of a [[Visual Studio Express|http://www.microsoft.com/express/]] project.
[[Download source for Acanthocephalan Proboscis Profiler GUI|https://sourceforge.net/projects/acanthocephala/files/]]


!Example datasets
If you don't yet have any data to analyse, you can try out Acanthocephalan Proboscis Profiler using these example datasets:

*[[Echinorhynchus spp. example data]]
*[[Echinorhynchus salmonis example data]]


!Description
Hook measurements from 36 female specimens of //Echinorhynchus salmonis// Müller, 1784 (Acanthocephala: Echinorhynchidae) from the Bothnian Bay, Baltic Sea. The acanthocephalans were collected from the sea spawning form of the whitefish //Coregonus lavaretus// L. and the smelt //Osmerus eperlanus// (L.) from the Bothnian Bay, northern Baltic Sea. For each specimen a row of hooks from the dorsal surface of the proboscis and a row of hooks from the ventral surface of the proboscis were measured. The specimens from which these data were collected are deposited in The Natural History Museum, London.

|!Host|!Number of specimens|!Voucher Specimens|
|//Coregonus lavaretus// L.|22|BMNH 2002.2.4.132-226|
|//Osmerus eperlanus// (L.)|14|BMNH 2002.2.4.227-263|

!Download
[[Echinorhynchus_salmonis.csv|downloads/Echinorhynchus_salmonis.csv]]

First column of spreadsheet ('specimen') is the accession number, //e.g.// d17.15
The first letter indicates if the data came from a dorsal row ('d') or a ventral row (v).
The number before the full stop indicates the host of the acanthocephalan, either //C. lavaretus// (5) or //O. eperlanus// (17).
The number after the full stop is the specimen number.

!More information
For a detailed description of the material, please see the following publication:
Wayland, M.T., Gibson, D.I. & Sommerville, C. (2004) //Echinorhynchus salmonis// Müller, 1784 (Acanthocephala: Echinorhynchidae) from the Bothnian Bay, Baltic Sea: morphological variability and radial asymmetry of proboscis hooks. //Systematic Parasitology//, ''58'':149-158. ([[download| http://www.springerlink.com/content/k8584611t1934583/]])
!Description

|!Species|!Host|!Locality|!Number of specimens|!Voucher Specimens|!id in data file|
|//E. gadi// sp. A|//Gadus morhua// L.|northern North Sea|4|n/a|a0*|
|//E. gadi// sp. B|//Gadus morhua// L.|northern North Sea|3|n/a|b01-b03|
|//E. gadi// sp. B|//Melanogrammus aeglefinus// (L.)|northern North Sea|1|n/a|b04|
|//E. truttae//|//Salmo trutta// L. |Drummore, southeast coast of Scotland|35|BM(NH) 1986.764-793|t1.*|
|//E. truttae//|//Salmo trutta// L. |Loch Walton Burn, River Carron catchment, central Scotland (National Grid Reference NS 668 865)|3|BM(NH) 2002.2.4.264-275|t2.*|
|//E. truttae//|//Salmo trutta// L. |Loch Coulter Burn, River Carron catchment, central Scotland (National Grid Reference NS 761 865|8|BM(NH) 2002.2.4.276-283|t3.*|



!Download
[[Echinorhynchus_spp.csv|downloads/Echinorhynchus_spp.csv]]

!More information
The molecular and morphometric characterization of //E. gadi// spp. A and B is described in the following paper:
Wayland MT, Gibson DI, Sommerville C. (2005) Morphometric discrimination of two allozymically diagnosed sibling species of the //Echinorhynchus gadi//  Zoega in Müller complex (Acanthocephala) in the North Sea. //Systematic Parasitology//, ''60'':139-149. ([[download|http://www.springerlink.com/content/t7164qq50222688w/]])
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!Plot Dendrogram Dialog
This dialog box enables you to control how a dendrogram of the hook profile data is plotted. 

[img[Plot Dendrogram Dialog|images/dendrogram_dialog.JPG]]


!!!Options
''Title'' - Title for graph (default is //Cluster Dendrogram//).

''Title Size'' - Magnification to be used for the graph title (default is 1)

''Leaf Label'' - There are three choices for the leaf labels: 
#group name and specimen unique identifier 
#specimen unique identifier only
#group name only

''Leaf Label Size'' - Magnification to be used for the leaf labels (default is 1)

''Y Axis Label'' - An optional label for the Y Axis of the graph (default is //Distance//)

''Axis Label Size'' - Magnification to be used for the axis label (default is 1)


!Dendrogram Window
The dendrogram is generated in an R graphics window:

[img[Dendrogram|images/dendrogram.JPG]]

This window can be resized. Other options are listed below.

!!!Save Graph to File
Select ''File'' -> ''Save as'' and then choose one of the following file types:
*Metafile
*Postscript
*PDF
*Png
*Bmp
*TIFF
*Jpeg

!!!Copy Graph to the Clipboard
Select ''File'' -> ''Copy to the clipboard'' and then choose one of these two options:
#as a bitmap
#as a metafile

!!!Print Graph
Select ''File'' -> ''Print''


!Overview
The main form displays six tabbed spreadsheets:
* Raw data
* Hook Length Profiles
* Hook Base Profiles
* PCA Scores
* PCA Loadings
* PC Importance (variance explained by each PC)

[img[Main Form|images/main_form.JPG]]

!File Menu
''Export Data'' - export Raw, Length Profiles, Base Profiles, All Profiles, PCA Scores or PCA Loadings
''New Analysis'' - start new analysis (discard current results)
''Exit'' - exit application

!Edit Menu
''Select All'' - select all data in visible spreadsheet.
''Copy'' - copy current selection

!Graph Menu
''Dendrogram'' - see [[GUI - Dendrogram]]
''Hook Metrics'' - [[GUI - Plot Hook Metrics]]
''PCA Scores'' - [[GUI - Plot PCA Scores]]
''PCA Loadings'' - [[GUI - Plot PCA Loadings]]

!Help Menu
''Online Help''
!Plot Hook Metrics Dialog
This dialog box enables you to control how the hook profile data are plotted.

[img[Plot Hook Metrics Dialog|images/plot_hook_metrics_dialog.JPG]]

!!!Options
''Data'' - choose either //raw// (original hook measurements) or //profiles// (metrics generated by moving average routine).

''Measurement'' - Select hook metric to plot from:
*Length
*Base

''Title Size'' - Magnification to be used for the graph title (default is 1)

''Symbol Size'' - Magnification to be used for the graph symbols (default is 1)

''Axis Label Size'' - Magnification to be used for the axis labels (default is 1)

''Tic Label Size'' - Magnification to be used for the tic labels (default is 1)

''Legend'' - Position of legend; choose one of the following options:
*no legend
*bottomright
*bottom
*bottomleft
*left
*topleft
*top
*topright
*right
*center

''Legend Size'' - Magnification to be used for the legend (default is 1)

''Group Colour'' - Select a colour from the drop-down list for each group

''Group Symbol'' - Select a symbol from the drop-down list for each group

''Identify Points'' - Check this box if you would like to be able to identify points on the graph by clicking on them.

!Hook Metric Plot Window
The plot is generated in an R graphics window.

[img[Profile Plot|images/profile_plot.JPG]]

This window can be resized. Other options are listed below.

!!!Identify points
If you checked the //Identify Points// box (see above) you can identify which specimen a plotted point belongs to, simply by clicking on it. Once you have finished identifying points, select ''Stop'' -> ''Stop locator''; you will then be able to access the other menu options (see below).

!!!Save Graph to File
Select ''File'' -> ''Save as'' and then choose one of the following file types:
*Metafile
*Postscript
*PDF
*Png
*Bmp
*TIFF
*Jpeg

!!!Copy Graph to the Clipboard
Select ''File'' -> ''Copy to the clipboard'' and then choose one of these two options:
#as a bitmap
#as a metafile

!!!Print Graph
Select ''File'' -> ''Print''



!Plot Principal Component Analysis (PCA) Scores
A simple PCA is performed when the proboscides are profiled. This dialog box enables you to plot the PCA loadings. The loadings define the size of the contribution of each original variable (hook measurement) to the PCs.

[img[Plot PCA loadings dialog|images/plot_pca_loadings_dialog.JPG]]

!!!Options
''X-axis'' - Principal Component (PC) to plot on x-axis

''Y-axis'' - PC to plot on y-axis

''Title'' - An optional title for the graph

''Title Size'' - Magnification to be used for the graph title (default is 1)

''Symbol Size'' - Magnification to be used for the graph symbols (default is 1)

''Axis Label Size'' - Magnification to be used for the axis labels (default is 1)

''Tic Label Size'' - Magnification to be used for the tic labels (default is 1)

!PCA Loadings Plot Window
The plot is generated in an R graphics window.

[img[PCA Loadings Plot|images/pca_loadings_plot.JPG]]

This window can be resized. Other options are listed below.

!!!Identify points
You can identify which variable (hook measurement) a plotted point belongs to, simply by clicking on it. Once you have finished identifying points, select ''Stop'' -> ''Stop locator''; you will then be able to access the other menu options (see below).

!!!Save Graph to File
Select ''File'' -> ''Save as'' and then choose one of the following file types:
*Metafile
*Postscript
*PDF
*Png
*Bmp
*TIFF
*Jpeg

!!!Copy Graph to the Clipboard
Select ''File'' -> ''Copy to the clipboard'' and then choose one of these two options:
#as a bitmap
#as a metafile

!!!Print Graph
Select ''File'' -> ''Print''



!Plot Principal Component Analysis (PCA) Scores
A simple PCA is performed when the proboscides are profiled. This dialog box enables you to plot the PCA scores.

[img[Plot PCA dialog|images/pca_dialog.JPG]]

!!!Options
''X-axis'' - Principal Component (PC) to plot on x-axis

''Y-axis'' - PC to plot on y-axis

''Title'' - An optional title for the graph

''Title Size'' - Magnification to be used for the graph title (default is 1)

''Symbol Size'' - Magnification to be used for the graph symbols (default is 1)

''Axis Label Size'' - Magnification to be used for the axis labels (default is 1)

''Tic Label Size'' - Magnification to be used for the tic labels (default is 1)

''Legend'' - Position of legend; choose one of the following options:
*no legend
*bottomright
*bottom
*bottomleft
*left
*topleft
*top
*topright
*right
*center

''Legend Size'' - Magnification to be used for the legend (default is 1)

''Group Colour'' - Select a colour from the drop-down list for each group

''Group Symbol'' - Select a symbol from the drop-down list for each group

''Identify Points'' - Check this box if you would like to be able to identify points on the graph by clicking on them.

!PCA Scores Plot Window
The plot is generated in an R graphics window.

[img[PCA Scores Plot|images/pca_scores_plot.JPG]]

This window can be resized. Other options are listed below.

!!!Identify points
If you checked the //Identify Points// box (see above) you can identify which specimen a plotted point belongs to, simply by clicking on it. Once you have finished identifying points, select ''Stop'' -> ''Stop locator''; you will then be able to access the other menu options (see below).

!!!Save Graph to File
Select ''File'' -> ''Save as'' and then choose one of the following file types:
*Metafile
*Postscript
*PDF
*Png
*Bmp
*TIFF
*Jpeg

!!!Copy Graph to the Clipboard
Select ''File'' -> ''Copy to the clipboard'' and then choose one of these two options:
#as a bitmap
#as a metafile

!!!Print Graph
Select ''File'' -> ''Print''



[img[Raw Data Form|images/raw_data_form.JPG]]

!Summary
This form displays the raw data that have been imported into the application. Notice that in addition to the data in the original import file (i.e. specimen, group, hook, length and base) an additional variable has been generated: 

{{{Position}}} - standardised position = position as counted from distal end of proboscis X 100 / n+1, where n = number of hooks in longitudinal row. 

!Options
!!!Export Data
To export data to a comma separated value (CSV) file, select ''File'' -> ''Export Data''

You will be prompted to give a file name and a location to store the file.
 
!!!Edit
Data cannot be changed, but they can be selected and copied.
To select all data use either the menu options:
''Edit'' -> ''Select All''
or use keyboard shortcut:
''Ctrl + A''

To copy selected data to the clipboard use either the menu options:
''Edit'' -> ''Copy''
or use the keyboard shortcut:
''Ctrl + C''

!!!Graph
To plot the raw data click ''Graph''.

This will open the [[Plot Hook Metrics Dialog|GUI - Plot Hook Metrics]]
When the application starts you will be presented with this form:

[img[form 1|images/form1.JPG]]

Click the ''Browse'' button to select a file of hook measurements (see [[Input File Format]] for detail of the type of file required) and then click ''Next'' to load the data.
Once the raw data are loaded the following form will be displayed:

[img[form 2|images/form2.JPG]]

This form provides a summary of the raw data. Clicking the ''View raw data'' button will open the [[Raw Data Form]] which displays the raw data and enables you to plot them and/or export them if required.

To generate the proboscis profiles select an appropriate moving average interval (the default is the minimum that can be applied to the dataset) and then click ''Next''.

Click ''Back'' button if you want to go back to the start page to load a different file of raw data.


!Data Handling
[[GUI - Raw Data Form]]
[[GUI - MainForm]]

!Graphics
[[GUI - Dendrogram]]
[[GUI - Plot Hook Metrics]]
[[GUI - Plot PCA Scores]]
[[GUI - Plot PCA Loadings]]
!Compatible operating systems
Acanthocephalan Proboscis Profiler GUI has been tested on Windows Vista and XP. It may also work on other Windows operating systems.

!Prerequisites
To be able to run the Acanthocephalan Proboscis Profiler GUI you need to have the following software installed on your computer:
*[[R|http://www.r-project.org/]]
*[[rscproxy|http://cran.r-project.org/web/packages/rscproxy/index.html]]
*[[DCOM|http://rcom.univie.ac.at/]]

[[R|http://www.r-project.org/]], [[rscproxy|http://cran.r-project.org/web/packages/rscproxy/index.html]] and [[DCOM|http://rcom.univie.ac.at/]] should be installed according to the following instructions (administrator privileges required):
#Download the latest version of [[R|http://www.r-project.org/]] and install accepting default options.
# A default installation of R provides two versions: 32bit (R i386) and 64bit (R x64). Acanthocephalan Proboscis profiler works with the 32bit version. Right click on either the start menu entry or desktop shortcut labelled "R i386" and select "Run as administrator" from the pop-up menu. When running R as an administrator, packages will be installed in the system library directory, rather than your user directory. The package rscproxy must be installed in the system library.
#Install rscproxy. You can do this using the packages menu or you may find it easier to run this command in the terminal: install.packages("rscproxy")
# Finally install DCOM which is available here: [[http://rcom.univie.ac.at/download/current/statconnDCOM3.6-0B3_Noncommercial.exe|http://rcom.univie.ac.at/download/current/statconnDCOM3.6-0B3_Noncommercial.exe]]
# Your computer is now ready to install the Acanthocephalan Proboscis Profiler GUI


!ClickOnce Installer
The Acanthocephalan Proboscis Profiler GUI is deployed using the [[ClickOnce|http://en.wikipedia.org/wiki/ClickOnce]] installer which does not require administrator privileges. The application is installed per-user rather than per-machine. Click following link to install:

[[INSTALL|install/setup.exe]]

!GUI Updates
If your computer is connected to the internet the Acanthocephalan Proboscis Profiler GUI will automatically check for updates. If a new version of the software is available, you will see the following prompt asking if you would like to upgrade.

[img[Update Available Dialog|images/update_available.JPG]]
!1 Install Application
See [[GUI Installation]]

!2 Run Application
Launch the application:
''Start Menu'' -> ''All Programs'' -> ''Acanthocephala'' -> ''Acanthocephalan Proboscis Profiler''

When the application starts you will be presented with this form:

[img[form 1|images/form1.JPG]]

Click the ''Browse'' button to select a file of hook measurements (see [[Input File Format]] for detail of the type of file required) and then click ''Next'' to load the data.

!3 Raw data loaded
Once the raw data are loaded the following form will be displayed:

[img[form 2|images/form2.JPG]]

This form provides a summary of the raw data. Clicking the ''View raw data'' button will open the [[Raw Data Form]] which displays the raw data and enables you to plot them if required.

To generate the proboscis profiles click ''Next''. The default value for the moving average interval is the minimum value that can be applied to the data. The default moving average interval works well for the data-sets tested, but you can choose a different value if you wish. 

!4 Profiles
Once the profiles have been computed the main form for the application will open, along with a dendrogram displaying the results of the cluster analysis.

!!!Dendrogram

[img[Dendrogram|images/dendrogram.JPG]]

The appearance of the dendrogram can be customized, see [[GUI - Dendrogram]]

!!!Main Form

[img[Main Form|images/main_form.JPG]]

The main form displays both the raw and profile data. For more information on the menu options see [[GUI - MainForm]]
/***
|Name:|HideWhenPlugin|
|Description:|Allows conditional inclusion/exclusion in templates|
|Version:|3.1 ($Rev: 3919 $)|
|Date:|$Date: 2008-03-13 02:03:12 +1000 (Thu, 13 Mar 2008) $|
|Source:|http://mptw.tiddlyspot.com/#HideWhenPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
For use in ViewTemplate and EditTemplate. Example usage:
{{{<div macro="showWhenTagged Task">[[TaskToolbar]]</div>}}}
{{{<div macro="showWhen tiddler.modifier == 'BartSimpson'"><img src="bart.gif"/></div>}}}
***/
//{{{

window.hideWhenLastTest = false;

window.removeElementWhen = function(test,place) {
	window.hideWhenLastTest = test;
	if (test) {
		removeChildren(place);
		place.parentNode.removeChild(place);
	}
};


merge(config.macros,{

	hideWhen: { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( eval(paramString), place);
	}},

	showWhen: { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( !eval(paramString), place);
	}},

	hideWhenTagged: { handler: function (place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( tiddler.tags.containsAll(params), place);
	}},

	showWhenTagged: { handler: function (place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( !tiddler.tags.containsAll(params), place);
	}},

	hideWhenTaggedAny: { handler: function (place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( tiddler.tags.containsAny(params), place);
	}},

	showWhenTaggedAny: { handler: function (place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( !tiddler.tags.containsAny(params), place);
	}},

	hideWhenTaggedAll: { handler: function (place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( tiddler.tags.containsAll(params), place);
	}},

	showWhenTaggedAll: { handler: function (place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( !tiddler.tags.containsAll(params), place);
	}},

	hideWhenExists: { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( store.tiddlerExists(params[0]) || store.isShadowTiddler(params[0]), place);
	}},

	showWhenExists: { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( !(store.tiddlerExists(params[0]) || store.isShadowTiddler(params[0])), place);
	}},

	hideWhenTitleIs: { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( tiddler.title == params[0], place);
	}},

	showWhenTitleIs: { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( tiddler.title != params[0], place);
	}},

	'else': { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
		removeElementWhen( !window.hideWhenLastTest, place);
	}}

});

//}}}

!Armature of the acanthocephalan proboscis
[img[SEM of acanthocephalan proboscis with one longitudinal row of hooks highlighted.|images/long_row_hooks.png]]

Hooks are arranged in longitudinal rows. One longitudinal row is highlighted in the image above. To create a 'proboscis profile' we need measurements from each of the hooks in at least one longitudinal row of hooks per specimen.

!Preparation of material
On removal from the host, acanthocephalans should be washed and relaxed in distilled water, then fixed in 70% ethanol. All acanthocephalans should be prepared for light microscopy using the same protocol. I recommend dehydrating the specimens in an alcohol series and then clearing and mounting them in lactophenol.

!Light microscopy
Select a longitudinal row in which all hooks are visible in profile.

[img[Line drawing of one longitudinal row of hooks in profile|images/brayi_hooks.png]]

!Measurements
From each hook in your selected row record two measurements: length (L) and base (B).
[img[Length and base measurements|images/hook_measurements.png]]

Hook measurements should be entered into a spreadsheet, see [[Input File Format]]
The input file for Acanthocephalan Proboscis Profiler should be a comma separated value (CSV) file with 5 columns: specimen, group, hook, length and base.

{{{specimen}}} - unique identifier for the specimen (or row of hooks if more than one row was measured per specimen)
{{{group}}} - name of group 
{{{hook}}} - numerical position of hook in longitudinal row as counted from the distal end of the probocis
{{{length}}} - length of hook blade
{{{base}}} - width of hook base

For instructions on collecting data, see [[How to Measure Hooks]]

Data can be entered into your favourite spreadsheet program and saved as a comma separated value (CSV) file:

[img[spreadsheet of raw data|images/data_format.JPG]]

Example data files are available:
*[[Echinorhynchus spp. example data]]
*[[Echinorhynchus salmonis example data]]
/***
|Name:|InstantTimestampPlugin|
|Description:|A handy way to insert timestamps in your tiddler content|
|Version:|1.0.10 ($Rev: 3646 $)|
|Date:|$Date: 2008-02-27 02:34:38 +1000 (Wed, 27 Feb 2008) $|
|Source:|http://mptw.tiddlyspot.com/#InstantTimestampPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
!!Usage
If you enter {ts} in your tiddler content (without the spaces) it will be replaced with a timestamp when you save the tiddler. Full list of formats:
* {ts} or {t} -> timestamp
* {ds} or {d} -> datestamp
* !ts or !t at start of line -> !!timestamp
* !ds or !d at start of line -> !!datestamp
(I added the extra ! since that's how I like it. Remove it from translations below if required)
!!Notes
* Change the timeFormat and dateFormat below to suit your preference.
* See also http://mptw2.tiddlyspot.com/#AutoCorrectPlugin
* You could invent other translations and add them to the translations array below.
***/
//{{{

config.InstantTimestamp = {

	// adjust to suit
	timeFormat: 'DD/0MM/YY 0hh:0mm',
	dateFormat: 'DD/0MM/YY',

	translations: [
		[/^!ts?$/img,  "'!!{{ts{'+now.formatString(config.InstantTimestamp.timeFormat)+'}}}'"],
		[/^!ds?$/img,  "'!!{{ds{'+now.formatString(config.InstantTimestamp.dateFormat)+'}}}'"],

		// thanks Adapted Cat
		[/\{ts?\}(?!\}\})/ig,"'{{ts{'+now.formatString(config.InstantTimestamp.timeFormat)+'}}}'"],
		[/\{ds?\}(?!\}\})/ig,"'{{ds{'+now.formatString(config.InstantTimestamp.dateFormat)+'}}}'"]
		
	],

	excludeTags: [
		"noAutoCorrect",
		"noTimestamp",
		"html",
		"CSS",
		"css",
		"systemConfig",
		"systemConfigDisabled",
		"zsystemConfig",
		"Plugins",
		"Plugin",
		"plugins",
		"plugin",
		"javascript",
		"code",
		"systemTheme",
		"systemPalette"
	],

	excludeTiddlers: [
		"StyleSheet",
		"StyleSheetLayout",
		"StyleSheetColors",
		"StyleSheetPrint"
		// more?
	]

}; 

TiddlyWiki.prototype.saveTiddler_mptw_instanttimestamp = TiddlyWiki.prototype.saveTiddler;
TiddlyWiki.prototype.saveTiddler = function(title,newTitle,newBody,modifier,modified,tags,fields,clearChangeCount,created) {

	tags = tags ? tags : []; // just in case tags is null
	tags = (typeof(tags) == "string") ? tags.readBracketedList() : tags;
	var conf = config.InstantTimestamp;

	if ( !tags.containsAny(conf.excludeTags) && !conf.excludeTiddlers.contains(newTitle) ) {

		var now = new Date();
		var trans = conf.translations;
		for (var i=0;i<trans.length;i++) {
			newBody = newBody.replace(trans[i][0], eval(trans[i][1]));
		}
	}

	// TODO: use apply() instead of naming all args?
	return this.saveTiddler_mptw_instanttimestamp(title,newTitle,newBody,modifier,modified,tags,fields,clearChangeCount,created);
}

// you can override these in StyleSheet 
setStylesheet(".ts,.ds { font-style:italic; }","instantTimestampStyles");

//}}}

[>img[SEM of proboscis of Echinorhynchus truttae|images/truttae.png]]
The phylum [[Acanthocephala|http://en.wikipedia.org/wiki/Acanthocephala]] is comprised of over a thousand described species of bilaterally symmetrical, dioecious, pseudocoelomate worms. They are characterized by the presence of a retractable proboscis armed with rows of recurved hooks which provides the means of attachment to the definitive host's gut wall. Acanthocephalans lack an alimentary canal and food is absorbed directly through the body wall from the host. Acanthocephalan development requires at least two hosts, an arthropod intermediate host and a vertebrate definitive host. All major groups of vertebrates are parasitized by the Acanthocephala, although infections of agnathan and elasmobranch fishes are rare.

The Acanthocephala are a relatively homogenous group, displaying a limited number of features which can provide useful taxonomic characters. Hook morphometrics are of key significance in the discrimination of closely related species of acanthocephalans. Subtle differences in proboscis armature are often the only morphological characteristics which will distinguish congeneric species. The hooks covering the acanthocephalan proboscis are not uniform in size and shape; a longitudinal row of hooks will typically show morphometric variation from apex to base and radial asymmetry of hooks is not uncommon.

Molecular studies are revealing a previously unrecognized diversity in this phylum. Several nominal species have been shown to represent complexes of morphologically cryptic biological species, making the analysis of ecological data and the search for unknown intermediate hosts problematical.The Acanthocephalan Proboscis Profiler was designed to detect morphological heterogeneity in collections of morphologically similar acanthocephalan worms based on the multivariate statistical analysis of proboscis hooks. The Acanthocephalan Proboscis Profiler identifies objective, natural groups in a collection of worms which may correspond to distinct biological species or populations. Initial analyses have shown that the acanthocephalan proboscis profiler can discriminate biological species of the //Echinorhynchus gadi// complex and differentiate between dorsal and ventral hook rows from the proboscis of //Echinorhynchus salmonis//.

The [[algorithm|Algorithm]] is inspired by the meristogram of Huffman & Bullock [Huffman DG & Bullock WL: Meristograms: graphical analysis of serial variation of proboscis hooks of //Echinorhynchus// (Acanthocephala) Systematic Zoology 1975, 24:333-345]. 

/***
|Name:|LessBackupsPlugin|
|Description:|Intelligently limit the number of backup files you create|
|Version:|3.0.1 ($Rev: 2320 $)|
|Date:|$Date: 2007-06-18 22:37:46 +1000 (Mon, 18 Jun 2007) $|
|Source:|http://mptw.tiddlyspot.com/#LessBackupsPlugin|
|Author:|Simon Baird|
|Email:|simon.baird@gmail.com|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
!!Description
You end up with just backup one per year, per month, per weekday, per hour, minute, and second.  So total number won't exceed about 200 or so. Can be reduced by commenting out the seconds/minutes/hours line from modes array
!!Notes
Works in IE and Firefox only.  Algorithm by Daniel Baird. IE specific code by by Saq Imtiaz.
***/
//{{{

var MINS  = 60 * 1000;
var HOURS = 60 * MINS;
var DAYS  = 24 * HOURS;

if (!config.lessBackups) {
	config.lessBackups = {
		// comment out the ones you don't want or set config.lessBackups.modes in your 'tweaks' plugin
		modes: [
			["YYYY",  365*DAYS], // one per year for ever
			["MMM",   31*DAYS],  // one per month
			["ddd",   7*DAYS],   // one per weekday
			//["d0DD",  1*DAYS],   // one per day of month
			["h0hh",  24*HOURS], // one per hour
			["m0mm",  1*HOURS],  // one per minute
			["s0ss",  1*MINS],   // one per second
			["latest",0]         // always keep last version. (leave this).
		]
	};
}

window.getSpecialBackupPath = function(backupPath) {

	var now = new Date();

	var modes = config.lessBackups.modes;

	for (var i=0;i<modes.length;i++) {

		// the filename we will try
		var specialBackupPath = backupPath.replace(/(\.)([0-9]+\.[0-9]+)(\.html)$/,
				'$1'+now.formatString(modes[i][0]).toLowerCase()+'$3')

		// open the file
		try {
			if (config.browser.isIE) {
				var fsobject = new ActiveXObject("Scripting.FileSystemObject")
				var fileExists  = fsobject.FileExists(specialBackupPath);
				if (fileExists) {
					var fileObject = fsobject.GetFile(specialBackupPath);
					var modDate = new Date(fileObject.DateLastModified).valueOf();
				}
			}
			else {
				netscape.security.PrivilegeManager.enablePrivilege("UniversalXPConnect");
				var file = Components.classes["@mozilla.org/file/local;1"].createInstance(Components.interfaces.nsILocalFile);
				file.initWithPath(specialBackupPath);
				var fileExists = file.exists();
				if (fileExists) {
					var modDate = file.lastModifiedTime;
				}
			}
		}
		catch(e) {
			// give up
			return backupPath;
		}

		// expiry is used to tell if it's an 'old' one. Eg, if the month is June and there is a
		// June file on disk that's more than an month old then it must be stale so overwrite
		// note that "latest" should be always written because the expiration period is zero (see above)
		var expiry = new Date(modDate + modes[i][1]);
		if (!fileExists || now > expiry)
			return specialBackupPath;
	}
}

// hijack the core function
window.getBackupPath_mptw_orig = window.getBackupPath;
window.getBackupPath = function(localPath) {
	return getSpecialBackupPath(getBackupPath_mptw_orig(localPath));
}

//}}}

//{{{

                    GNU GENERAL PUBLIC LICENSE
                       Version 3, 29 June 2007

 Copyright (C) 2007 Free Software Foundation, Inc. <http://fsf.org/>
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in one of these ways:

    a) Convey the object code in, or embodied in, a physical product
    (including a physical distribution medium), accompanied by the
    Corresponding Source fixed on a durable physical medium
    customarily used for software interchange.

    b) Convey the object code in, or embodied in, a physical product
    (including a physical distribution medium), accompanied by a
    written offer, valid for at least three years and valid for as
    long as you offer spare parts or customer support for that product
    model, to give anyone who possesses the object code either (1) a
    copy of the Corresponding Source for all the software in the
    product that is covered by this License, on a durable physical
    medium customarily used for software interchange, for a price no
    more than your reasonable cost of physically performing this
    conveying of source, or (2) access to copy the
    Corresponding Source from a network server at no charge.

    c) Convey individual copies of the object code with a copy of the
    written offer to provide the Corresponding Source.  This
    alternative is allowed only occasionally and noncommercially, and
    only if you received the object code with such an offer, in accord
    with subsection 6b.

    d) Convey the object code by offering access from a designated
    place (gratis or for a charge), and offer equivalent access to the
    Corresponding Source in the same way through the same place at no
    further charge.  You need not require recipients to copy the
    Corresponding Source along with the object code.  If the place to
    copy the object code is a network server, the Corresponding Source
    may be on a different server (operated by you or a third party)
    that supports equivalent copying facilities, provided you maintain
    clear directions next to the object code saying where to find the
    Corresponding Source.  Regardless of what server hosts the
    Corresponding Source, you remain obligated to ensure that it is
    available for as long as needed to satisfy these requirements.

    e) Convey the object code using peer-to-peer transmission, provided
    you inform other peers where the object code and Corresponding
    Source of the work are being offered to the general public at no
    charge under subsection 6d.

  A separable portion of the object code, whose source code is excluded
from the Corresponding Source as a System Library, need not be
included in conveying the object code work.

  A "User Product" is either (1) a "consumer product", which means any
tangible personal property which is normally used for personal, family,
or household purposes, or (2) anything designed or sold for incorporation
into a dwelling.  In determining whether a product is a consumer product,
doubtful cases shall be resolved in favor of coverage.  For a particular
product received by a particular user, "normally used" refers to a
typical or common use of that class of product, regardless of the status
of the particular user or of the way in which the particular user
actually uses, or expects or is expected to use, the product.  A product
is a consumer product regardless of whether the product has substantial
commercial, industrial or non-consumer uses, unless such uses represent
the only significant mode of use of the product.

  "Installation Information" for a User Product means any methods,
procedures, authorization keys, or other information required to install
and execute modified versions of a covered work in that User Product from
a modified version of its Corresponding Source.  The information must
suffice to ensure that the continued functioning of the modified object
code is in no case prevented or interfered with solely because
modification has been made.

  If you convey an object code work under this section in, or with, or
specifically for use in, a User Product, and the conveying occurs as
part of a transaction in which the right of possession and use of the
User Product is transferred to the recipient in perpetuity or for a
fixed term (regardless of how the transaction is characterized), the
Corresponding Source conveyed under this section must be accompanied
by the Installation Information.  But this requirement does not apply
if neither you nor any third party retains the ability to install
modified object code on the User Product (for example, the work has
been installed in ROM).

  The requirement to provide Installation Information does not include a
requirement to continue to provide support service, warranty, or updates
for a work that has been modified or installed by the recipient, or for
the User Product in which it has been modified or installed.  Access to a
network may be denied when the modification itself materially and
adversely affects the operation of the network or violates the rules and
protocols for communication across the network.

  Corresponding Source conveyed, and Installation Information provided,
in accord with this section must be in a format that is publicly
documented (and with an implementation available to the public in
source code form), and must require no special password or key for
unpacking, reading or copying.

  7. Additional Terms.

  "Additional permissions" are terms that supplement the terms of this
License by making exceptions from one or more of its conditions.
Additional permissions that are applicable to the entire Program shall
be treated as though they were included in this License, to the extent
that they are valid under applicable law.  If additional permissions
apply only to part of the Program, that part may be used separately
under those permissions, but the entire Program remains governed by
this License without regard to the additional permissions.

  When you convey a copy of a covered work, you may at your option
remove any additional permissions from that copy, or from any part of
it.  (Additional permissions may be written to require their own
removal in certain cases when you modify the work.)  You may place
additional permissions on material, added by you to a covered work,
for which you have or can give appropriate copyright permission.

  Notwithstanding any other provision of this License, for material you
add to a covered work, you may (if authorized by the copyright holders of
that material) supplement the terms of this License with terms:

    a) Disclaiming warranty or limiting liability differently from the
    terms of sections 15 and 16 of this License; or

    b) Requiring preservation of specified reasonable legal notices or
    author attributions in that material or in the Appropriate Legal
    Notices displayed by works containing it; or

    c) Prohibiting misrepresentation of the origin of that material, or
    requiring that modified versions of such material be marked in
    reasonable ways as different from the original version; or

    d) Limiting the use for publicity purposes of names of licensors or
    authors of the material; or

    e) Declining to grant rights under trademark law for use of some
    trade names, trademarks, or service marks; or

    f) Requiring indemnification of licensors and authors of that
    material by anyone who conveys the material (or modified versions of
    it) with contractual assumptions of liability to the recipient, for
    any liability that these contractual assumptions directly impose on
    those licensors and authors.

  All other non-permissive additional terms are considered "further
restrictions" within the meaning of section 10.  If the Program as you
received it, or any part of it, contains a notice stating that it is
governed by this License along with a term that is a further
restriction, you may remove that term.  If a license document contains
a further restriction but permits relicensing or conveying under this
License, you may add to a covered work material governed by the terms
of that license document, provided that the further restriction does
not survive such relicensing or conveying.

  If you add terms to a covered work in accord with this section, you
must place, in the relevant source files, a statement of the
additional terms that apply to those files, or a notice indicating
where to find the applicable terms.

  Additional terms, permissive or non-permissive, may be stated in the
form of a separately written license, or stated as exceptions;
the above requirements apply either way.

  8. Termination.

  You may not propagate or modify a covered work except as expressly
provided under this License.  Any attempt otherwise to propagate or
modify it is void, and will automatically terminate your rights under
this License (including any patent licenses granted under the third
paragraph of section 11).

  However, if you cease all violation of this License, then your
license from a particular copyright holder is reinstated (a)
provisionally, unless and until the copyright holder explicitly and
finally terminates your license, and (b) permanently, if the copyright
holder fails to notify you of the violation by some reasonable means
prior to 60 days after the cessation.

  Moreover, your license from a particular copyright holder is
reinstated permanently if the copyright holder notifies you of the
violation by some reasonable means, this is the first time you have
received notice of violation of this License (for any work) from that
copyright holder, and you cure the violation prior to 30 days after
your receipt of the notice.

  Termination of your rights under this section does not terminate the
licenses of parties who have received copies or rights from you under
this License.  If your rights have been terminated and not permanently
reinstated, you do not qualify to receive new licenses for the same
material under section 10.

  9. Acceptance Not Required for Having Copies.

  You are not required to accept this License in order to receive or
run a copy of the Program.  Ancillary propagation of a covered work
occurring solely as a consequence of using peer-to-peer transmission
to receive a copy likewise does not require acceptance.  However,
nothing other than this License grants you permission to propagate or
modify any covered work.  These actions infringe copyright if you do
not accept this License.  Therefore, by modifying or propagating a
covered work, you indicate your acceptance of this License to do so.

  10. Automatic Licensing of Downstream Recipients.

  Each time you convey a covered work, the recipient automatically
receives a license from the original licensors, to run, modify and
propagate that work, subject to this License.  You are not responsible
for enforcing compliance by third parties with this License.

  An "entity transaction" is a transaction transferring control of an
organization, or substantially all assets of one, or subdividing an
organization, or merging organizations.  If propagation of a covered
work results from an entity transaction, each party to that
transaction who receives a copy of the work also receives whatever
licenses to the work the party's predecessor in interest had or could
give under the previous paragraph, plus a right to possession of the
Corresponding Source of the work from the predecessor in interest, if
the predecessor has it or can get it with reasonable efforts.

  You may not impose any further restrictions on the exercise of the
rights granted or affirmed under this License.  For example, you may
not impose a license fee, royalty, or other charge for exercise of
rights granted under this License, and you may not initiate litigation
(including a cross-claim or counterclaim in a lawsuit) alleging that
any patent claim is infringed by making, using, selling, offering for
sale, or importing the Program or any portion of it.

  11. Patents.

  A "contributor" is a copyright holder who authorizes use under this
License of the Program or a work on which the Program is based.  The
work thus licensed is called the contributor's "contributor version".

  A contributor's "essential patent claims" are all patent claims
owned or controlled by the contributor, whether already acquired or
hereafter acquired, that would be infringed by some manner, permitted
by this License, of making, using, or selling its contributor version,
but do not include claims that would be infringed only as a
consequence of further modification of the contributor version.  For
purposes of this definition, "control" includes the right to grant
patent sublicenses in a manner consistent with the requirements of
this License.

  Each contributor grants you a non-exclusive, worldwide, royalty-free
patent license under the contributor's essential patent claims, to
make, use, sell, offer for sale, import and otherwise run, modify and
propagate the contents of its contributor version.

  In the following three paragraphs, a "patent license" is any express
agreement or commitment, however denominated, not to enforce a patent
(such as an express permission to practice a patent or covenant not to
sue for patent infringement).  To "grant" such a patent license to a
party means to make such an agreement or commitment not to enforce a
patent against the party.

  If you convey a covered work, knowingly relying on a patent license,
and the Corresponding Source of the work is not available for anyone
to copy, free of charge and under the terms of this License, through a
publicly available network server or other readily accessible means,
then you must either (1) cause the Corresponding Source to be so
available, or (2) arrange to deprive yourself of the benefit of the
patent license for this particular work, or (3) arrange, in a manner
consistent with the requirements of this License, to extend the patent
license to downstream recipients.  "Knowingly relying" means you have
actual knowledge that, but for the patent license, your conveying the
covered work in a country, or your recipient's use of the covered work
in a country, would infringe one or more identifiable patents in that
country that you have reason to believe are valid.

  If, pursuant to or in connection with a single transaction or
arrangement, you convey, or propagate by procuring conveyance of, a
covered work, and grant a patent license to some of the parties
receiving the covered work authorizing them to use, propagate, modify
or convey a specific copy of the covered work, then the patent license
you grant is automatically extended to all recipients of the covered
work and works based on it.

  A patent license is "discriminatory" if it does not include within
the scope of its coverage, prohibits the exercise of, or is
conditioned on the non-exercise of one or more of the rights that are
specifically granted under this License.  You may not convey a covered
work if you are a party to an arrangement with a third party that is
in the business of distributing software, under which you make payment
to the third party based on the extent of your activity of conveying
the work, and under which the third party grants, to any of the
parties who would receive the covered work from you, a discriminatory
patent license (a) in connection with copies of the covered work
conveyed by you (or copies made from those copies), or (b) primarily
for and in connection with specific products or compilations that
contain the covered work, unless you entered into that arrangement,
or that patent license was granted, prior to 28 March 2007.

  Nothing in this License shall be construed as excluding or limiting
any implied license or other defenses to infringement that may
otherwise be available to you under applicable patent law.

  12. No Surrender of Others' Freedom.

  If conditions are imposed on you (whether by court order, agreement or
otherwise) that contradict the conditions of this License, they do not
excuse you from the conditions of this License.  If you cannot convey a
covered work so as to satisfy simultaneously your obligations under this
License and any other pertinent obligations, then as a consequence you may
not convey it at all.  For example, if you agree to terms that obligate you
to collect a royalty for further conveying from those to whom you convey
the Program, the only way you could satisfy both those terms and this
License would be to refrain entirely from conveying the Program.

  13. Use with the GNU Affero General Public License.

  Notwithstanding any other provision of this License, you have
permission to link or combine any covered work with a work licensed
under version 3 of the GNU Affero General Public License into a single
combined work, and to convey the resulting work.  The terms of this
License will continue to apply to the part which is the covered work,
but the special requirements of the GNU Affero General Public License,
section 13, concerning interaction through a network will apply to the
combination as such.

  14. Revised Versions of this License.

  The Free Software Foundation may publish revised and/or new versions of
the GNU General Public License from time to time.  Such new versions will
be similar in spirit to the present version, but may differ in detail to
address new problems or concerns.

  Each version is given a distinguishing version number.  If the
Program specifies that a certain numbered version of the GNU General
Public License "or any later version" applies to it, you have the
option of following the terms and conditions either of that numbered
version or of any later version published by the Free Software
Foundation.  If the Program does not specify a version number of the
GNU General Public License, you may choose any version ever published
by the Free Software Foundation.

  If the Program specifies that a proxy can decide which future
versions of the GNU General Public License can be used, that proxy's
public statement of acceptance of a version permanently authorizes you
to choose that version for the Program.

  Later license versions may give you additional or different
permissions.  However, no additional obligations are imposed on any
author or copyright holder as a result of your choosing to follow a
later version.

  15. Disclaimer of Warranty.

  THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY
APPLICABLE LAW.  EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT
HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY
OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO,
THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR
PURPOSE.  THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM
IS WITH YOU.  SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF
ALL NECESSARY SERVICING, REPAIR OR CORRECTION.

  16. Limitation of Liability.

  IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING
WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MODIFIES AND/OR CONVEYS
THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY
GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE
USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF
DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD
PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS),
EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF
SUCH DAMAGES.

  17. Interpretation of Sections 15 and 16.

  If the disclaimer of warranty and limitation of liability provided
above cannot be given local legal effect according to their terms,
reviewing courts shall apply local law that most closely approximates
an absolute waiver of all civil liability in connection with the
Program, unless a warranty or assumption of liability accompanies a
copy of the Program in return for a fee.

                     END OF TERMS AND CONDITIONS

            How to Apply These Terms to Your New Programs

  If you develop a new program, and you want it to be of the greatest
possible use to the public, the best way to achieve this is to make it
free software which everyone can redistribute and change under these terms.

  To do so, attach the following notices to the program.  It is safest
to attach them to the start of each source file to most effectively
state the exclusion of warranty; and each file should have at least
the "copyright" line and a pointer to where the full notice is found.

    <one line to give the program's name and a brief idea of what it does.>
    Copyright (C) <year>  <name of author>

    This program is free software: you can redistribute it and/or modify
    it under the terms of the GNU General Public License as published by
    the Free Software Foundation, either version 3 of the License, or
    (at your option) any later version.

    This program is distributed in the hope that it will be useful,
    but WITHOUT ANY WARRANTY; without even the implied warranty of
    MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
    GNU General Public License for more details.

    You should have received a copy of the GNU General Public License
    along with this program.  If not, see <http://www.gnu.org/licenses/>.

Also add information on how to contact you by electronic and paper mail.

  If the program does terminal interaction, make it output a short
notice like this when it starts in an interactive mode:

    <program>  Copyright (C) <year>  <name of author>
    This program comes with ABSOLUTELY NO WARRANTY; for details type `show w'.
    This is free software, and you are welcome to redistribute it
    under certain conditions; type `show c' for details.

The hypothetical commands `show w' and `show c' should show the appropriate
parts of the General Public License.  Of course, your program's commands
might be different; for a GUI interface, you would use an "about box".

  You should also get your employer (if you work as a programmer) or school,
if any, to sign a "copyright disclaimer" for the program, if necessary.
For more information on this, and how to apply and follow the GNU GPL, see
<http://www.gnu.org/licenses/>.

  The GNU General Public License does not permit incorporating your program
into proprietary programs.  If your program is a subroutine library, you
may consider it more useful to permit linking proprietary applications with
the library.  If this is what you want to do, use the GNU Lesser General
Public License instead of this License.  But first, please read
<http://www.gnu.org/philosophy/why-not-lgpl.html>.
//}}}
MPTW is a distribution or edition of TiddlyWiki that includes a standard TiddlyWiki core packaged with some plugins designed to improve usability and provide a better way to organise your information. For more information see http://mptw.tiddlyspot.com/.
[[Introduction]]
[[Algorithm]]
[[Documentation]]
[[Publications]]
[[Download]]
[[Contact]]
Name: MptwBlack
Background: #000
Foreground: #fff
PrimaryPale: #333
PrimaryLight: #555
PrimaryMid: #888
PrimaryDark: #aaa
SecondaryPale: #111
SecondaryLight: #222
SecondaryMid: #555
SecondaryDark: #888
TertiaryPale: #222
TertiaryLight: #666
TertiaryMid: #888
TertiaryDark: #aaa
Error: #300

This is in progress. Help appreciated.


Name: MptwBlue
Background: #fff
Foreground: #000
PrimaryPale: #cdf
PrimaryLight: #57c
PrimaryMid: #114
PrimaryDark: #012
SecondaryPale: #ffc
SecondaryLight: #fe8
SecondaryMid: #db4
SecondaryDark: #841
TertiaryPale: #eee
TertiaryLight: #ccc
TertiaryMid: #999
TertiaryDark: #666
Error: #f88

/***
|Name:|MptwConfigPlugin|
|Description:|Miscellaneous tweaks used by MPTW|
|Version:|1.0 ($Rev: 3646 $)|
|Date:|$Date: 2008-02-27 02:34:38 +1000 (Wed, 27 Feb 2008) $|
|Source:|http://mptw.tiddlyspot.com/#MptwConfigPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#MptwConfigPlugin|
!!Note: instead of editing this you should put overrides in MptwUserConfigPlugin
***/
//{{{
var originalReadOnly = readOnly;
var originalShowBackstage = showBackstage;

config.options.chkHttpReadOnly = false; 		// means web visitors can experiment with your site by clicking edit
readOnly = false;								// needed because the above doesn't work any more post 2.1 (??)
showBackstage = true;							// show backstage for same reason

config.options.chkInsertTabs = true;    		// tab inserts a tab when editing a tiddler
config.views.wikified.defaultText = "";			// don't need message when a tiddler doesn't exist
config.views.editor.defaultText = "";			// don't need message when creating a new tiddler 

config.options.chkSaveBackups = true;			// do save backups
config.options.txtBackupFolder = 'twbackup';	// put backups in a backups folder

config.options.chkAutoSave = (window.location.protocol == "file:"); // do autosave if we're in local file

config.mptwVersion = "2.5.3";

config.macros.mptwVersion={handler:function(place){wikify(config.mptwVersion,place);}};

if (config.options.txtTheme == '')
	config.options.txtTheme = 'MptwTheme';

// add to default GettingStarted
config.shadowTiddlers.GettingStarted += "\n\nSee also [[MPTW]].";

// add select theme and palette controls in default OptionsPanel
config.shadowTiddlers.OptionsPanel = config.shadowTiddlers.OptionsPanel.replace(/(\n\-\-\-\-\nAlso see AdvancedOptions)/, "{{select{<<selectTheme>>\n<<selectPalette>>}}}$1");

// these are used by ViewTemplate
config.mptwDateFormat = 'DD/MM/YY';
config.mptwJournalFormat = 'Journal DD/MM/YY';

//}}}
Name: MptwGreen
Background: #fff
Foreground: #000
PrimaryPale: #9b9
PrimaryLight: #385
PrimaryMid: #031
PrimaryDark: #020
SecondaryPale: #ffc
SecondaryLight: #fe8
SecondaryMid: #db4
SecondaryDark: #841
TertiaryPale: #eee
TertiaryLight: #ccc
TertiaryMid: #999
TertiaryDark: #666
Error: #f88

Name: MptwRed
Background: #fff
Foreground: #000
PrimaryPale: #eaa
PrimaryLight: #c55
PrimaryMid: #711
PrimaryDark: #500
SecondaryPale: #ffc
SecondaryLight: #fe8
SecondaryMid: #db4
SecondaryDark: #841
TertiaryPale: #eee
TertiaryLight: #ccc
TertiaryMid: #999
TertiaryDark: #666
Error: #f88

|Name|MptwRounded|
|Description|Mptw Theme with some rounded corners (Firefox only)|
|ViewTemplate|MptwTheme##ViewTemplate|
|EditTemplate|MptwTheme##EditTemplate|
|PageTemplate|MptwTheme##PageTemplate|
|StyleSheet|##StyleSheet|

!StyleSheet
/*{{{*/

[[MptwTheme##StyleSheet]]

.tiddler,
.sliderPanel,
.button,
.tiddlyLink,
.tabContents
{ -moz-border-radius: 1em; }

.tab {
	-moz-border-radius-topleft: 0.5em;
	-moz-border-radius-topright: 0.5em;
}
#topMenu {
	-moz-border-radius-bottomleft: 2em;
	-moz-border-radius-bottomright: 2em;
}

/*}}}*/

Name: MptwSmoke
Background: #fff
Foreground: #000
PrimaryPale: #aaa
PrimaryLight: #777
PrimaryMid: #111
PrimaryDark: #000
SecondaryPale: #ffc
SecondaryLight: #fe8
SecondaryMid: #db4
SecondaryDark: #841
TertiaryPale: #eee
TertiaryLight: #ccc
TertiaryMid: #999
TertiaryDark: #666
Error: #f88

|Name|MptwStandard|
|Description|Mptw Theme with the default TiddlyWiki PageLayout and Styles|
|ViewTemplate|MptwTheme##ViewTemplate|
|EditTemplate|MptwTheme##EditTemplate|
Name: MptwTeal
Background: #fff
Foreground: #000
PrimaryPale: #B5D1DF
PrimaryLight: #618FA9
PrimaryMid: #1a3844
PrimaryDark: #000
SecondaryPale: #ffc
SecondaryLight: #fe8
SecondaryMid: #db4
SecondaryDark: #841
TertiaryPale: #f8f8f8
TertiaryLight: #bbb
TertiaryMid: #999
TertiaryDark: #888
Error: #f88
|Name|MptwTheme|
|Description|Mptw Theme including custom PageLayout|
|PageTemplate|##PageTemplate|
|ViewTemplate|##ViewTemplate|
|EditTemplate|##EditTemplate|
|StyleSheet|##StyleSheet|

http://mptw.tiddlyspot.com/#MptwTheme ($Rev: 1829 $)

!PageTemplate
<!--{{{-->
<div class='header' macro='gradient vert [[ColorPalette::PrimaryLight]] [[ColorPalette::PrimaryMid]]'>
	<div class='headerShadow'>
		<span class='siteTitle' refresh='content' tiddler='SiteTitle'></span>&nbsp;
		<span class='siteSubtitle' refresh='content' tiddler='SiteSubtitle'></span>
	</div>
	<div class='headerForeground'>
		<span class='siteTitle' refresh='content' tiddler='SiteTitle'></span>&nbsp;
		<span class='siteSubtitle' refresh='content' tiddler='SiteSubtitle'></span>
	</div>
</div>
<!-- horizontal MainMenu -->
<div id='topMenu' refresh='content' tiddler='MainMenu'></div>
<!-- original MainMenu menu -->
<!-- <div id='mainMenu' refresh='content' tiddler='MainMenu'></div> -->
<div id='sidebar'>
	<div id='sidebarOptions' refresh='content' tiddler='SideBarOptions'></div>
	<div id='sidebarTabs' refresh='content' force='true' tiddler='SideBarTabs'></div>
</div>
<div id='displayArea'>
	<div id='messageArea'></div>
	<div id='tiddlerDisplay'></div>
</div>
<!--}}}-->

!ViewTemplate
<!--{{{-->
[[MptwTheme##ViewTemplateToolbar]]

<div class="tagglyTagged" macro="tags"></div>

<div class='titleContainer'>
	<span class='title' macro='view title'></span>
	<span macro="miniTag"></span>
</div>

<div class='subtitle'>
	(updated <span macro='view modified date {{config.mptwDateFormat?config.mptwDateFormat:"MM/0DD/YY"}}'></span>
	by <span macro='view modifier link'></span>)
	<!--
	(<span macro='message views.wikified.createdPrompt'></span>
	<span macro='view created date {{config.mptwDateFormat?config.mptwDateFormat:"MM/0DD/YY"}}'></span>)
	-->
</div>

<div macro="showWhen tiddler.tags.containsAny(['css','html','pre','systemConfig']) && !tiddler.text.match('{{'+'{')">
	<div class='viewer'><pre macro='view text'></pre></div>
</div>
<div macro="else">
	<div class='viewer' macro='view text wikified'></div>
</div>

<div class="tagglyTagging" macro="tagglyTagging"></div>

<!--}}}-->

!ViewTemplateToolbar
<!--{{{-->
<div class='toolbar'>
	<span macro="showWhenTagged systemConfig">
		<span macro="toggleTag systemConfigDisable . '[[disable|systemConfigDisable]]'"></span>
	</span>
	<span macro="showWhenTagged systemTheme"><span macro="applyTheme"></span></span>
	<span macro="showWhenTagged systemPalette"><span macro="applyPalette"></span></span>
	<span macro="showWhen tiddler.tags.contains('css') || tiddler.title == 'StyleSheet'"><span macro="refreshAll"></span></span>
	<span style="padding:1em;"></span>
	<span macro='toolbar closeTiddler closeOthers +editTiddler deleteTiddler > fields syncing permalink references jump'></span> <span macro='newHere label:"new here"'></span>
	<span macro='newJournalHere {{config.mptwJournalFormat?config.mptwJournalFormat:"MM/0DD/YY"}}'></span>
</div>
<!--}}}-->

!EditTemplate
<!--{{{-->
<div class="toolbar" macro="toolbar +saveTiddler saveCloseTiddler closeOthers -cancelTiddler cancelCloseTiddler deleteTiddler"></div>
<div class="title" macro="view title"></div>
<div class="editLabel">Title</div><div class="editor" macro="edit title"></div>
<div macro='annotations'></div>
<div class="editLabel">Content</div><div class="editor" macro="edit text"></div>
<div class="editLabel">Tags</div><div class="editor" macro="edit tags"></div>
<div class="editorFooter"><span macro="message views.editor.tagPrompt"></span><span macro="tagChooser"></span></div>
<!--}}}-->

!StyleSheet
/*{{{*/

/* a contrasting background so I can see where one tiddler ends and the other begins */
body {
	background: [[ColorPalette::TertiaryLight]];
}

/* sexy colours and font for the header */
.headerForeground {
	color: [[ColorPalette::PrimaryPale]];
}
.headerShadow, .headerShadow a {
	color: [[ColorPalette::PrimaryMid]];
}

/* separate the top menu parts */
.headerForeground, .headerShadow {
	padding: 1em 1em 0;
}

.headerForeground, .headerShadow {
	font-family: 'Trebuchet MS' sans-serif;
	font-weight:bold;
}
.headerForeground .siteSubtitle {
	color: [[ColorPalette::PrimaryLight]];
}
.headerShadow .siteSubtitle {
	color: [[ColorPalette::PrimaryMid]];
}

/* make shadow go and down right instead of up and left */
.headerShadow {
	left: 1px;
	top: 1px;
}

/* prefer monospace for editing */
.editor textarea, .editor input {
	font-family: 'Consolas' monospace;
	background-color:[[ColorPalette::TertiaryPale]];
}


/* sexy tiddler titles */
.title {
	font-size: 250%;
	color: [[ColorPalette::PrimaryLight]];
	font-family: 'Trebuchet MS' sans-serif;
}

/* more subtle tiddler subtitle */
.subtitle {
	padding:0px;
	margin:0px;
	padding-left:1em;
	font-size: 90%;
	color: [[ColorPalette::TertiaryMid]];
}
.subtitle .tiddlyLink {
	color: [[ColorPalette::TertiaryMid]];
}

/* a little bit of extra whitespace */
.viewer {
	padding-bottom:3px;
}

/* don't want any background color for headings */
h1,h2,h3,h4,h5,h6 {
	background-color: transparent;
	color: [[ColorPalette::Foreground]];
}

/* give tiddlers 3d style border and explicit background */
.tiddler {
	background: [[ColorPalette::Background]];
	border-right: 2px [[ColorPalette::TertiaryMid]] solid;
	border-bottom: 2px [[ColorPalette::TertiaryMid]] solid;
	margin-bottom: 1em;
	padding:1em 2em 2em 1.5em;
}

/* make options slider look nicer */
#sidebarOptions .sliderPanel {
	border:solid 1px [[ColorPalette::PrimaryLight]];
}

/* the borders look wrong with the body background */
#sidebar .button {
	border-style: none;
}

/* this means you can put line breaks in SidebarOptions for readability */
#sidebarOptions br {
	display:none;
}
/* undo the above in OptionsPanel */
#sidebarOptions .sliderPanel br {
	display:inline;
}

/* horizontal main menu stuff */
#displayArea {
	margin: 1em 15.7em 0em 1em; /* use the freed up space */
}
#topMenu br {
	display: none;
}
#topMenu {
	background: [[ColorPalette::PrimaryMid]];
	color:[[ColorPalette::PrimaryPale]];
}
#topMenu {
	padding:2px;
}
#topMenu .button, #topMenu .tiddlyLink, #topMenu a {
	margin-left: 0.5em;
	margin-right: 0.5em;
	padding-left: 3px;
	padding-right: 3px;
	color: [[ColorPalette::PrimaryPale]];
	font-size: 115%;
}
#topMenu .button:hover, #topMenu .tiddlyLink:hover {
	background: [[ColorPalette::PrimaryDark]];
}

/* make 2.2 act like 2.1 with the invisible buttons */
.toolbar {
	visibility:hidden;
}
.selected .toolbar {
	visibility:visible;
}

/* experimental. this is a little borked in IE7 with the button 
 * borders but worth it I think for the extra screen realestate */
.toolbar { float:right; }

/* fix for TaggerPlugin. from sb56637. improved by FND */
.popup li .tagger a {
   display:inline;
}

/* makes theme selector look a little better */
#sidebarOptions .sliderPanel .select .button {
  padding:0.5em;
  display:block;
}
#sidebarOptions .sliderPanel .select br {
	display:none;
}

/* make it print a little cleaner */
@media print {
	#topMenu {
		display: none ! important;
	}
	/* not sure if we need all the importants */
	.tiddler {
		border-style: none ! important;
		margin:0px ! important;
		padding:0px ! important;
		padding-bottom:2em ! important;
	}
	.tagglyTagging .button, .tagglyTagging .hidebutton {
		display: none ! important;
	}
	.headerShadow {
		visibility: hidden ! important;
	}
	.tagglyTagged .quickopentag, .tagged .quickopentag {
		border-style: none ! important;
	}
	.quickopentag a.button, .miniTag {
		display: none ! important;
	}
}

/* get user styles specified in StyleSheet */
[[StyleSheet]]

/*}}}*/

|Name|MptwTrim|
|Description|Mptw Theme with a reduced header to increase useful space|
|ViewTemplate|MptwTheme##ViewTemplate|
|EditTemplate|MptwTheme##EditTemplate|
|StyleSheet|MptwTheme##StyleSheet|
|PageTemplate|##PageTemplate|

!PageTemplate
<!--{{{-->

<!-- horizontal MainMenu -->
<div id='topMenu' macro='gradient vert [[ColorPalette::PrimaryLight]] [[ColorPalette::PrimaryMid]]'>
<span refresh='content' tiddler='SiteTitle' style="padding-left:1em;font-weight:bold;"></span>:
<span refresh='content' tiddler='MainMenu'></span>
</div>
<div id='sidebar'>
	<div id='sidebarOptions'>
		<div refresh='content' tiddler='SideBarOptions'></div>
		<div style="margin-left:0.1em;"
			macro='slider chkTabSliderPanel SideBarTabs {{"tabs \u00bb"}} "Show Timeline, All, Tags, etc"'></div>
	</div>
</div>
<div id='displayArea'>
	<div id='messageArea'></div>
	<div id='tiddlerDisplay'></div>
</div>

For upgrading. See [[ImportTiddlers]].
URL: http://mptw.tiddlyspot.com/upgrade.html
/***
|Description:|A place to put your config tweaks so they aren't overwritten when you upgrade MPTW|
See http://www.tiddlywiki.org/wiki/Configuration_Options for other options you can set. In some cases where there are clashes with other plugins it might help to rename this to zzMptwUserConfigPlugin so it gets executed last.
***/
//{{{

// example: set your preferred date format
//config.mptwDateFormat = 'MM/0DD/YY';
//config.mptwJournalFormat = 'Journal MM/0DD/YY';

// example: set the theme you want to start with
//config.options.txtTheme = 'MptwRoundTheme';

// example: switch off autosave, switch on backups and set a backup folder
//config.options.chkSaveBackups = true;
//config.options.chkAutoSave = false;
//config.options.txtBackupFolder = 'backups';

// uncomment to disable 'new means new' functionality for the new journal macro
//config.newMeansNewForJournalsToo = false;

//}}}
/***
|Name:|NewHerePlugin|
|Description:|Creates the new here and new journal macros|
|Version:|3.0 ($Rev: 3861 $)|
|Date:|$Date: 2008-03-08 10:53:09 +1000 (Sat, 08 Mar 2008) $|
|Source:|http://mptw.tiddlyspot.com/#NewHerePlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License|http://mptw.tiddlyspot.com/#TheBSDLicense|
***/
//{{{
merge(config.macros, {
	newHere: {
		handler: function(place,macroName,params,wikifier,paramString,tiddler) {
			wikify("<<newTiddler "+paramString+" tag:[["+tiddler.title+"]]>>",place,null,tiddler);
		}
	},
	newJournalHere: {
		handler: function(place,macroName,params,wikifier,paramString,tiddler) {
			wikify("<<newJournal "+paramString+" tag:[["+tiddler.title+"]]>>",place,null,tiddler);
		}
	}
});

//}}}

/***
|Name:|NewMeansNewPlugin|
|Description:|If 'New Tiddler' already exists then create 'New Tiddler (1)' and so on|
|Version:|1.1.1 ($Rev: 2263 $)|
|Date:|$Date: 2007-06-13 04:22:32 +1000 (Wed, 13 Jun 2007) $|
|Source:|http://mptw.tiddlyspot.com/empty.html#NewMeansNewPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License|http://mptw.tiddlyspot.com/#TheBSDLicense|
!!Note: I think this should be in the core
***/
//{{{

// change this or set config.newMeansNewForJournalsToo it in MptwUuserConfigPlugin
if (config.newMeansNewForJournalsToo == undefined) config.newMeansNewForJournalsToo = true;

String.prototype.getNextFreeName = function() {
       var numberRegExp = / \(([0-9]+)\)$/;
       var match = numberRegExp.exec(this);
       if (match) {
               var num = parseInt(match[1]) + 1;
               return this.replace(numberRegExp," ("+num+")");
       }
       else {
               return this + " (1)";
       }
}

config.macros.newTiddler.checkForUnsaved = function(newName) {
	var r = false;
	story.forEachTiddler(function(title,element) {
		if (title == newName)
			r = true;
	});
	return r;
}

config.macros.newTiddler.getName = function(newName) {
       while (store.getTiddler(newName) || config.macros.newTiddler.checkForUnsaved(newName))
               newName = newName.getNextFreeName();
       return newName;
}


config.macros.newTiddler.onClickNewTiddler = function()
{
	var title = this.getAttribute("newTitle");
	if(this.getAttribute("isJournal") == "true") {
		title = new Date().formatString(title.trim());
	}

	// ---- these three lines should be the only difference between this and the core onClickNewTiddler
	if (config.newMeansNewForJournalsToo || this.getAttribute("isJournal") != "true")
		title = config.macros.newTiddler.getName(title);

	var params = this.getAttribute("params");
	var tags = params ? params.split("|") : [];
	var focus = this.getAttribute("newFocus");
	var template = this.getAttribute("newTemplate");
	var customFields = this.getAttribute("customFields");
	if(!customFields && !store.isShadowTiddler(title))
		customFields = String.encodeHashMap(config.defaultCustomFields);
	story.displayTiddler(null,title,template,false,null,null);
	var tiddlerElem = story.getTiddler(title);
	if(customFields)
		story.addCustomFields(tiddlerElem,customFields);
	var text = this.getAttribute("newText");
	if(typeof text == "string")
		story.getTiddlerField(title,"text").value = text.format([title]);
	for(var t=0;t<tags.length;t++)
		story.setTiddlerTag(title,tags[t],+1);
	story.focusTiddler(title,focus);
	return false;
};

//}}}

/***
|Name:|PrettyDatesPlugin|
|Description:|Provides a new date format ('pppp') that displays times such as '2 days ago'|
|Version:|1.0 ($Rev: 3646 $)|
|Date:|$Date: 2008-02-27 02:34:38 +1000 (Wed, 27 Feb 2008) $|
|Source:|http://mptw.tiddlyspot.com/#PrettyDatesPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
!!Notes
* If you want to you can rename this plugin. :) Some suggestions: LastUpdatedPlugin, RelativeDatesPlugin, SmartDatesPlugin, SexyDatesPlugin.
* Inspired by http://ejohn.org/files/pretty.js
***/
//{{{
Date.prototype.prettyDate = function() {
	var diff = (((new Date()).getTime() - this.getTime()) / 1000);
	var day_diff = Math.floor(diff / 86400);

	if (isNaN(day_diff))      return "";
	else if (diff < 0)        return "in the future";
	else if (diff < 60)       return "just now";
	else if (diff < 120)      return "1 minute ago";
	else if (diff < 3600)     return Math.floor(diff/60) + " minutes ago";
	else if (diff < 7200)     return "1 hour ago";
	else if (diff < 86400)    return Math.floor(diff/3600) + " hours ago";
	else if (day_diff == 1)   return "Yesterday";
	else if (day_diff < 7)    return day_diff + " days ago";
	else if (day_diff < 14)   return  "a week ago";
	else if (day_diff < 31)   return Math.ceil(day_diff/7) + " weeks ago";
	else if (day_diff < 62)   return "a month ago";
	else if (day_diff < 365)  return "about " + Math.ceil(day_diff/31) + " months ago";
	else if (day_diff < 730)  return "a year ago";
	else                      return Math.ceil(day_diff/365) + " years ago";
}

Date.prototype.formatString_orig_mptw = Date.prototype.formatString;

Date.prototype.formatString = function(template) {
	return this.formatString_orig_mptw(template).replace(/pppp/,this.prettyDate());
}

// for MPTW. otherwise edit your ViewTemplate as required.
// config.mptwDateFormat = 'pppp (DD/MM/YY)'; 
config.mptwDateFormat = 'pppp'; 

//}}}

!Studies that have used Acanthocephalan Proboscis Profiler

[[Alcántar-Escalera, F. J., García-Varela, M., Vázquez-Domínguez, E. and Pérez-Ponce de León, G. (2013), Using DNA barcoding to link cystacanths and adults of the acanthocephalan Polymorphus brevis in central Mexico. Molecular Ecology Resources, 13: 1116–1124. | http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12090/abstract]]

[[Wayland M (2013) Morphological variation in Echinorhynchus truttae Schrank, 1788 and the E. bothniensis Zdzitowiecki & Valtonen, 1987 species complex from freshwater fishes of northern Europe. Biodiversity Data Journal 1: e975. | http://dx.doi.org/10.3897/BDJ.1.e975]]

!Original publication describing the algorithm
[[Wayland MT. Proboscis profiler: a tool for detecting acanthocephalan morphotypes. Systematic Parasitology 2010 Jul;76(3):159-67. | http://link.springer.com/article/10.1007/s11230-010-9245-z]]

Reprints available on request (e-mail: mwayland[at]users.sourceforge.net).
/***
|Name:|QuickOpenTagPlugin|
|Description:|Changes tag links to make it easier to open tags as tiddlers|
|Version:|3.0.1 ($Rev: 3861 $)|
|Date:|$Date: 2008-03-08 10:53:09 +1000 (Sat, 08 Mar 2008) $|
|Source:|http://mptw.tiddlyspot.com/#QuickOpenTagPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
***/
//{{{
config.quickOpenTag = {

	dropdownChar: (document.all ? "\u25bc" : "\u25be"), // the little one doesn't work in IE?

	createTagButton: function(place,tag,excludeTiddler) {
		// little hack so we can do this: <<tag PrettyTagName|RealTagName>>
		var splitTag = tag.split("|");
		var pretty = tag;
		if (splitTag.length == 2) {
			tag = splitTag[1];
			pretty = splitTag[0];
		}
		
		var sp = createTiddlyElement(place,"span",null,"quickopentag");
		createTiddlyText(createTiddlyLink(sp,tag,false),pretty);
		
		var theTag = createTiddlyButton(sp,config.quickOpenTag.dropdownChar,
                        config.views.wikified.tag.tooltip.format([tag]),onClickTag);
		theTag.setAttribute("tag",tag);
		if (excludeTiddler)
			theTag.setAttribute("tiddler",excludeTiddler);
    		return(theTag);
	},

	miniTagHandler: function(place,macroName,params,wikifier,paramString,tiddler) {
		var tagged = store.getTaggedTiddlers(tiddler.title);
		if (tagged.length > 0) {
			var theTag = createTiddlyButton(place,config.quickOpenTag.dropdownChar,
                        	config.views.wikified.tag.tooltip.format([tiddler.title]),onClickTag);
			theTag.setAttribute("tag",tiddler.title);
			theTag.className = "miniTag";
		}
	},

	allTagsHandler: function(place,macroName,params) {
		var tags = store.getTags(params[0]);
		var filter = params[1]; // new feature
		var ul = createTiddlyElement(place,"ul");
		if(tags.length == 0)
			createTiddlyElement(ul,"li",null,"listTitle",this.noTags);
		for(var t=0; t<tags.length; t++) {
			var title = tags[t][0];
			if (!filter || (title.match(new RegExp('^'+filter)))) {
				var info = getTiddlyLinkInfo(title);
				var theListItem =createTiddlyElement(ul,"li");
				var theLink = createTiddlyLink(theListItem,tags[t][0],true);
				var theCount = " (" + tags[t][1] + ")";
				theLink.appendChild(document.createTextNode(theCount));
				var theDropDownBtn = createTiddlyButton(theListItem," " +
					config.quickOpenTag.dropdownChar,this.tooltip.format([tags[t][0]]),onClickTag);
				theDropDownBtn.setAttribute("tag",tags[t][0]);
			}
		}
	},

	// todo fix these up a bit
	styles: [
"/*{{{*/",
"/* created by QuickOpenTagPlugin */",
".tagglyTagged .quickopentag, .tagged .quickopentag ",
"	{ margin-right:1.2em; border:1px solid #eee; padding:2px; padding-right:0px; padding-left:1px; }",
".quickopentag .tiddlyLink { padding:2px; padding-left:3px; }",
".quickopentag a.button { padding:1px; padding-left:2px; padding-right:2px;}",
"/* extra specificity to make it work right */",
"#displayArea .viewer .quickopentag a.button, ",
"#displayArea .viewer .quickopentag a.tiddyLink, ",
"#mainMenu .quickopentag a.tiddyLink, ",
"#mainMenu .quickopentag a.tiddyLink ",
"	{ border:0px solid black; }",
"#displayArea .viewer .quickopentag a.button, ",
"#mainMenu .quickopentag a.button ",
"	{ margin-left:0px; padding-left:2px; }",
"#displayArea .viewer .quickopentag a.tiddlyLink, ",
"#mainMenu .quickopentag a.tiddlyLink ",
"	{ margin-right:0px; padding-right:0px; padding-left:0px; margin-left:0px; }",
"a.miniTag {font-size:150%;} ",
"#mainMenu .quickopentag a.button ",
"	/* looks better in right justified main menus */",
"	{ margin-left:0px; padding-left:2px; margin-right:0px; padding-right:0px; }", 
"#topMenu .quickopentag { padding:0px; margin:0px; border:0px; }",
"#topMenu .quickopentag .tiddlyLink { padding-right:1px; margin-right:0px; }",
"#topMenu .quickopentag .button { padding-left:1px; margin-left:0px; border:0px; }",
"/*}}}*/",
		""].join("\n"),

	init: function() {
		// we fully replace these builtins. can't hijack them easily
		window.createTagButton = this.createTagButton;
		config.macros.allTags.handler = this.allTagsHandler;
		config.macros.miniTag = { handler: this.miniTagHandler };
		config.shadowTiddlers["QuickOpenTagStyles"] = this.styles;
		store.addNotification("QuickOpenTagStyles",refreshStyles);
	}
}

config.quickOpenTag.init();

//}}}

to do
/***
|Name:|RenameTagsPlugin|
|Description:|Allows you to easily rename or delete tags across multiple tiddlers|
|Version:|3.0 ($Rev: 5501 $)|
|Date:|$Date: 2008-06-10 23:11:55 +1000 (Tue, 10 Jun 2008) $|
|Source:|http://mptw.tiddlyspot.com/#RenameTagsPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License|http://mptw.tiddlyspot.com/#TheBSDLicense|
Rename a tag and you will be prompted to rename it in all its tagged tiddlers.
***/
//{{{
config.renameTags = {

	prompts: {
		rename: "Rename the tag '%0' to '%1' in %2 tidder%3?",
		remove: "Remove the tag '%0' from %1 tidder%2?"
	},

	removeTag: function(tag,tiddlers) {
		store.suspendNotifications();
		for (var i=0;i<tiddlers.length;i++) {
			store.setTiddlerTag(tiddlers[i].title,false,tag);
		}
		store.resumeNotifications();
		store.notifyAll();
	},

	renameTag: function(oldTag,newTag,tiddlers) {
		store.suspendNotifications();
		for (var i=0;i<tiddlers.length;i++) {
			store.setTiddlerTag(tiddlers[i].title,false,oldTag); // remove old
			store.setTiddlerTag(tiddlers[i].title,true,newTag);  // add new
		}
		store.resumeNotifications();
		store.notifyAll();
	},

	storeMethods: {

		saveTiddler_orig_renameTags: TiddlyWiki.prototype.saveTiddler,

		saveTiddler: function(title,newTitle,newBody,modifier,modified,tags,fields,clearChangeCount,created) {
			if (title != newTitle) {
				var tagged = this.getTaggedTiddlers(title);
				if (tagged.length > 0) {
					// then we are renaming a tag
					if (confirm(config.renameTags.prompts.rename.format([title,newTitle,tagged.length,tagged.length>1?"s":""])))
						config.renameTags.renameTag(title,newTitle,tagged);

					if (!this.tiddlerExists(title) && newBody == "")
						// dont create unwanted tiddler
						return null;
				}
			}
			return this.saveTiddler_orig_renameTags(title,newTitle,newBody,modifier,modified,tags,fields,clearChangeCount,created);
		},

		removeTiddler_orig_renameTags: TiddlyWiki.prototype.removeTiddler,

		removeTiddler: function(title) {
			var tagged = this.getTaggedTiddlers(title);
			if (tagged.length > 0)
				if (confirm(config.renameTags.prompts.remove.format([title,tagged.length,tagged.length>1?"s":""])))
					config.renameTags.removeTag(title,tagged);
			return this.removeTiddler_orig_renameTags(title);
		}

	},

	init: function() {
		merge(TiddlyWiki.prototype,this.storeMethods);
	}
}

config.renameTags.init();

//}}}

/***
|Name:|SaveCloseTiddlerPlugin|
|Description:|Provides two extra toolbar commands, saveCloseTiddler and cancelCloseTiddler|
|Version:|3.0 ($Rev: 5502 $)|
|Date:|$Date: 2008-06-10 23:31:39 +1000 (Tue, 10 Jun 2008) $|
|Source:|http://mptw.tiddlyspot.com/#SaveCloseTiddlerPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
To use these you must add them to the tool bar in your EditTemplate
***/
//{{{
merge(config.commands,{

	saveCloseTiddler: {
		text: 'done/close',
		tooltip: 'Save changes to this tiddler and close it',
		handler: function(ev,src,title) {
			var closeTitle = title;
			var newTitle = story.saveTiddler(title,ev.shiftKey);
			if (newTitle)
				closeTitle = newTitle;
			return config.commands.closeTiddler.handler(ev,src,closeTitle);
		}
	},

	cancelCloseTiddler: {
		text: 'cancel/close',
		tooltip: 'Undo changes to this tiddler and close it',
		handler: function(ev,src,title) {
			// the same as closeTiddler now actually
			return config.commands.closeTiddler.handler(ev,src,title);
		}
	}

});

//}}}

/***
|Name:|SelectThemePlugin|
|Description:|Lets you easily switch theme and palette|
|Version:|1.0.1 ($Rev: 3646 $)|
|Date:|$Date: 2008-02-27 02:34:38 +1000 (Wed, 27 Feb 2008) $|
|Source:|http://mptw.tiddlyspot.com/#SelectThemePlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
!Notes
* Borrows largely from ThemeSwitcherPlugin by Martin Budden http://www.martinswiki.com/#ThemeSwitcherPlugin
* Theme is cookie based. But set a default by setting config.options.txtTheme in MptwConfigPlugin (for example)
* Palette is not cookie based. It actually overwrites your ColorPalette tiddler when you select a palette, so beware. 
!Usage
* {{{<<selectTheme>>}}} makes a dropdown selector
* {{{<<selectPalette>>}}} makes a dropdown selector
* {{{<<applyTheme>>}}} applies the current tiddler as a theme
* {{{<<applyPalette>>}}} applies the current tiddler as a palette
* {{{<<applyTheme TiddlerName>>}}} applies TiddlerName as a theme
* {{{<<applyPalette TiddlerName>>}}} applies TiddlerName as a palette
***/
//{{{

config.macros.selectTheme = {
	label: {
      		selectTheme:"select theme",
      		selectPalette:"select palette"
	},
	prompt: {
		selectTheme:"Select the current theme",
		selectPalette:"Select the current palette"
	},
	tags: {
		selectTheme:'systemTheme',
		selectPalette:'systemPalette'
	}
};

config.macros.selectTheme.handler = function(place,macroName)
{
	var btn = createTiddlyButton(place,this.label[macroName],this.prompt[macroName],this.onClick);
	// want to handle palettes and themes with same code. use mode attribute to distinguish
	btn.setAttribute('mode',macroName);
};

config.macros.selectTheme.onClick = function(ev)
{
	var e = ev ? ev : window.event;
	var popup = Popup.create(this);
	var mode = this.getAttribute('mode');
	var tiddlers = store.getTaggedTiddlers(config.macros.selectTheme.tags[mode]);
	// for default
	if (mode == "selectPalette") {
		var btn = createTiddlyButton(createTiddlyElement(popup,'li'),"(default)","default color palette",config.macros.selectTheme.onClickTheme);
		btn.setAttribute('theme',"(default)");
		btn.setAttribute('mode',mode);
	}
	for(var i=0; i<tiddlers.length; i++) {
		var t = tiddlers[i].title;
		var name = store.getTiddlerSlice(t,'Name');
		var desc = store.getTiddlerSlice(t,'Description');
		var btn = createTiddlyButton(createTiddlyElement(popup,'li'), name?name:t, desc?desc:config.macros.selectTheme.label['mode'], config.macros.selectTheme.onClickTheme);
		btn.setAttribute('theme',t);
		btn.setAttribute('mode',mode);
	}
	Popup.show();
	return stopEvent(e);
};

config.macros.selectTheme.onClickTheme = function(ev)
{
	var mode = this.getAttribute('mode');
	var theme = this.getAttribute('theme');
	if (mode == 'selectTheme')
		story.switchTheme(theme);
	else // selectPalette
		config.macros.selectTheme.updatePalette(theme);
	return false;
};

config.macros.selectTheme.updatePalette = function(title)
{
	if (title != "") {
		store.deleteTiddler("ColorPalette");
		if (title != "(default)")
			store.saveTiddler("ColorPalette","ColorPalette",store.getTiddlerText(title),
					config.options.txtUserName,undefined,"");
		refreshAll();
		if(config.options.chkAutoSave)
			saveChanges(true);
	}
};

config.macros.applyTheme = {
	label: "apply",
	prompt: "apply this theme or palette" // i'm lazy
};

config.macros.applyTheme.handler = function(place,macroName,params,wikifier,paramString,tiddler) {
	var useTiddler = params[0] ? params[0] : tiddler.title;
	var btn = createTiddlyButton(place,this.label,this.prompt,config.macros.selectTheme.onClickTheme);
	btn.setAttribute('theme',useTiddler);
	btn.setAttribute('mode',macroName=="applyTheme"?"selectTheme":"selectPalette"); // a bit untidy here
}

config.macros.selectPalette = config.macros.selectTheme;
config.macros.applyPalette = config.macros.applyTheme;

config.macros.refreshAll = { handler: function(place,macroName,params,wikifier,paramString,tiddler) {
	createTiddlyButton(place,"refresh","refresh layout and styles",function() { refreshAll(); });
}};

//}}}

A tool for detecting acanthocephalan morphotypes
Acanthocephalan Proboscis Profiler
<<allTags excludeLists>>
<<tabs txtMoreTab "Tags" "All Tags" TabAllTags "Miss" "Missing tiddlers" TabMoreMissing "Orph" "Orphaned tiddlers" TabMoreOrphans "Shad" "Shadowed tiddlers" TabMoreShadowed>>
<<allTags excludeLists [a-z]>>
/***
|Name:|TagglyTaggingPlugin|
|Description:|tagglyTagging macro is a replacement for the builtin tagging macro in your ViewTemplate|
|Version:|3.3.1 ($Rev: 9828 $)|
|Date:|$Date: 2009-06-03 21:38:41 +1000 (Wed, 03 Jun 2009) $|
|Source:|http://mptw.tiddlyspot.com/#TagglyTaggingPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
!Notes
See http://mptw.tiddlyspot.com/#TagglyTagging
***/
//{{{

merge(String.prototype,{

	parseTagExpr: function(debug) {

		if (this.trim() == "")
			return "(true)";

		var anyLogicOp = /(!|&&|\|\||\(|\))/g;
		var singleLogicOp = /^(!|&&|\|\||\(|\))$/;

		var spaced = this.
			// because square brackets in templates are no good
			// this means you can use [(With Spaces)] instead of [[With Spaces]]
			replace(/\[\(/g," [[").
			replace(/\)\]/g,"]] "). 
			// space things out so we can use readBracketedList. tricky eh?
			replace(anyLogicOp," $1 ");

		var expr = "";

		var tokens = spaced.readBracketedList(false); // false means don't uniq the list. nice one JR!

		for (var i=0;i<tokens.length;i++)
			if (tokens[i].match(singleLogicOp))
				expr += tokens[i];
			else
				expr += "tiddler.tags.contains('%0')".format([tokens[i].replace(/'/,"\\'")]); // fix single quote bug. still have round bracket bug i think

		if (debug)
			alert(expr);

		return '('+expr+')';
	}

});

merge(TiddlyWiki.prototype,{
	getTiddlersByTagExpr: function(tagExpr,sortField) {

		var result = [];

		var expr = tagExpr.parseTagExpr();

		store.forEachTiddler(function(title,tiddler) {
			if (eval(expr))
				result.push(tiddler);
		});

		if(!sortField)
			sortField = "title";

		result.sort(function(a,b) {return a[sortField] < b[sortField] ? -1 : (a[sortField] == b[sortField] ? 0 : +1);});
		
		return result;
	}
});

config.taggly = {

	// for translations
	lingo: {
		labels: {
			asc:        "\u2191", // down arrow
			desc:       "\u2193", // up arrow
			title:      "title",
			modified:   "modified",
			created:    "created",
			show:       "+",
			hide:       "-",
			normal:     "normal",
			group:      "group",
			commas:     "commas",
			sitemap:    "sitemap",
			numCols:    "cols\u00b1", // plus minus sign
			label:      "Tagged as '%0':",
			exprLabel:  "Matching tag expression '%0':",
			excerpts:   "excerpts",
			descr:      "descr",
			slices:     "slices",
			contents:   "contents",
			sliders:    "sliders",
			noexcerpts: "title only",
			noneFound:  "(none)"
		},

		tooltips: {
			title:      "Click to sort by title",
			modified:   "Click to sort by modified date",
			created:    "Click to sort by created date",
			show:       "Click to show tagging list",
			hide:       "Click to hide tagging list",
			normal:     "Click to show a normal ungrouped list",
			group:      "Click to show list grouped by tag",
			sitemap:    "Click to show a sitemap style list",
			commas:     "Click to show a comma separated list",
			numCols:    "Click to change number of columns",
			excerpts:   "Click to show excerpts",
			descr:      "Click to show the description slice",
			slices:     "Click to show all slices",
			contents:   "Click to show entire tiddler contents",
			sliders:    "Click to show tiddler contents in sliders",
			noexcerpts: "Click to show entire title only"
		},

		tooDeepMessage: "* //sitemap too deep...//"
	},

	config: {
		showTaggingCounts: true,
		listOpts: {
			// the first one will be the default
			sortBy:     ["title","modified","created"],
			sortOrder:  ["asc","desc"],
			hideState:  ["show","hide"],
			listMode:   ["normal","group","sitemap","commas"],
			numCols:    ["1","2","3","4","5","6"],
			excerpts:   ["noexcerpts","excerpts","descr","slices","contents","sliders"]
		},
		valuePrefix: "taggly.",
		excludeTags: ["excludeLists","excludeTagging"],
		excerptSize: 50,
		excerptMarker: "/%"+"%/",
		siteMapDepthLimit: 25
	},

	getTagglyOpt: function(title,opt) {
		var val = store.getValue(title,this.config.valuePrefix+opt);
		return val ? val : this.config.listOpts[opt][0];
	},

	setTagglyOpt: function(title,opt,value) {
		// create it silently if it doesn't exist
		if (!store.tiddlerExists(title)) {
			store.saveTiddler(title,title,config.views.editor.defaultText.format([title]),config.options.txtUserName,new Date(),"");

			// <<tagglyTagging expr:"...">> creates a tiddler to store its display settings
			// Make those tiddlers less noticeable by tagging as excludeSearch and excludeLists
			// Because we don't want to hide real tags, check that they aren't actually tags before doing so
			// Also tag them as tagglyExpression for manageability
			// (contributed by RA)
			if (!store.getTaggedTiddlers(title).length) {
				store.setTiddlerTag(title,true,"excludeSearch");
				store.setTiddlerTag(title,true,"excludeLists");
				store.setTiddlerTag(title,true,"tagglyExpression");
			}
		}

		// if value is default then remove it to save space
		return store.setValue(title, this.config.valuePrefix+opt, value == this.config.listOpts[opt][0] ? null : value);
	},

	getNextValue: function(title,opt) {
		var current = this.getTagglyOpt(title,opt);
		var pos = this.config.listOpts[opt].indexOf(current);
		// supposed to automagically don't let cols cycle up past the number of items
		// currently broken in some situations, eg when using an expression
		// lets fix it later when we rewrite for jquery
		// the columns thing should be jquery table manipulation probably
		var limit = (opt == "numCols" ? store.getTaggedTiddlers(title).length : this.config.listOpts[opt].length);
		var newPos = (pos + 1) % limit;
		return this.config.listOpts[opt][newPos];
	},

	toggleTagglyOpt: function(title,opt) {
		var newVal = this.getNextValue(title,opt);
		this.setTagglyOpt(title,opt,newVal);
	}, 

	createListControl: function(place,title,type) {
		var lingo = config.taggly.lingo;
		var label;
		var tooltip;
		var onclick;

		if ((type == "title" || type == "modified" || type == "created")) {
			// "special" controls. a little tricky. derived from sortOrder and sortBy
			label = lingo.labels[type];
			tooltip = lingo.tooltips[type];

			if (this.getTagglyOpt(title,"sortBy") == type) {
				label += lingo.labels[this.getTagglyOpt(title,"sortOrder")];
				onclick = function() {
					config.taggly.toggleTagglyOpt(title,"sortOrder");
					return false;
				}
			}
			else {
				onclick = function() {
					config.taggly.setTagglyOpt(title,"sortBy",type);
					config.taggly.setTagglyOpt(title,"sortOrder",config.taggly.config.listOpts.sortOrder[0]);
					return false;
				}
			}
		}
		else {
			// "regular" controls, nice and simple
			label = lingo.labels[type == "numCols" ? type : this.getNextValue(title,type)];
			tooltip = lingo.tooltips[type == "numCols" ? type : this.getNextValue(title,type)];
			onclick = function() {
				config.taggly.toggleTagglyOpt(title,type);
				return false;
			}
		}

		// hide button because commas don't have columns
		if (!(this.getTagglyOpt(title,"listMode") == "commas" && type == "numCols"))
			createTiddlyButton(place,label,tooltip,onclick,type == "hideState" ? "hidebutton" : "button");
	},

	makeColumns: function(orig,numCols) {
		var listSize = orig.length;
		var colSize = listSize/numCols;
		var remainder = listSize % numCols;

		var upperColsize = colSize;
		var lowerColsize = colSize;

		if (colSize != Math.floor(colSize)) {
			// it's not an exact fit so..
			upperColsize = Math.floor(colSize) + 1;
			lowerColsize = Math.floor(colSize);
		}

		var output = [];
		var c = 0;
		for (var j=0;j<numCols;j++) {
			var singleCol = [];
			var thisSize = j < remainder ? upperColsize : lowerColsize;
			for (var i=0;i<thisSize;i++) 
				singleCol.push(orig[c++]);
			output.push(singleCol);
		}

		return output;
	},

	drawTable: function(place,columns,theClass) {
		var newTable = createTiddlyElement(place,"table",null,theClass);
		var newTbody = createTiddlyElement(newTable,"tbody");
		var newTr = createTiddlyElement(newTbody,"tr");
		for (var j=0;j<columns.length;j++) {
			var colOutput = "";
			for (var i=0;i<columns[j].length;i++) 
				colOutput += columns[j][i];
			var newTd = createTiddlyElement(newTr,"td",null,"tagglyTagging"); // todo should not need this class
			wikify(colOutput,newTd);
		}
		return newTable;
	},

	createTagglyList: function(place,title,isTagExpr) {
		switch(this.getTagglyOpt(title,"listMode")) {
			case "group":  return this.createTagglyListGrouped(place,title,isTagExpr); break;
			case "normal": return this.createTagglyListNormal(place,title,false,isTagExpr); break;
			case "commas": return this.createTagglyListNormal(place,title,true,isTagExpr); break;
			case "sitemap":return this.createTagglyListSiteMap(place,title,isTagExpr); break;
		}
	},

	getTaggingCount: function(title,isTagExpr) {
		// thanks to Doug Edmunds
		if (this.config.showTaggingCounts) {
			var tagCount = config.taggly.getTiddlers(title,'title',isTagExpr).length;
			if (tagCount > 0)
				return " ("+tagCount+")";
		}
		return "";
	},

	getTiddlers: function(titleOrExpr,sortBy,isTagExpr) {
		return isTagExpr ? store.getTiddlersByTagExpr(titleOrExpr,sortBy) : store.getTaggedTiddlers(titleOrExpr,sortBy);
	},

	getExcerpt: function(inTiddlerTitle,title,indent) {
		if (!indent)
			indent = 1;

		var displayMode = this.getTagglyOpt(inTiddlerTitle,"excerpts");
		var t = store.getTiddler(title);

		if (t && displayMode == "excerpts") {
			var text = t.text.replace(/\n/," ");
			var marker = text.indexOf(this.config.excerptMarker);
			if (marker != -1) {
				return " {{excerpt{<nowiki>" + text.substr(0,marker) + "</nowiki>}}}";
			}
			else if (text.length < this.config.excerptSize) {
				return " {{excerpt{<nowiki>" + t.text + "</nowiki>}}}";
			}
			else {
				return " {{excerpt{<nowiki>" + t.text.substr(0,this.config.excerptSize) + "..." + "</nowiki>}}}";
			}
		}
		else if (t && displayMode == "contents") {
			return "\n{{contents indent"+indent+"{\n" + t.text + "\n}}}";
		}
		else if (t && displayMode == "sliders") {
			return "<slider slide>\n{{contents{\n" + t.text + "\n}}}\n</slider>";
		}
		else if (t && displayMode == "descr") {
			var descr = store.getTiddlerSlice(title,'Description');
			return descr ? " {{excerpt{" + descr  + "}}}" : "";
		}
		else if (t && displayMode == "slices") {
			var result = "";
			var slices = store.calcAllSlices(title);
			for (var s in slices)
				result += "|%0|<nowiki>%1</nowiki>|\n".format([s,slices[s]]);
			return result ? "\n{{excerpt excerptIndent{\n" + result  + "}}}" : "";
		}
		return "";
	},

	notHidden: function(t,inTiddler) {
		if (typeof t == "string") 
			t = store.getTiddler(t);
		return (!t || !t.tags.containsAny(this.config.excludeTags) ||
				(inTiddler && this.config.excludeTags.contains(inTiddler)));
	},

	// this is for normal and commas mode
	createTagglyListNormal: function(place,title,useCommas,isTagExpr) {

		var list = config.taggly.getTiddlers(title,this.getTagglyOpt(title,"sortBy"),isTagExpr);

		if (this.getTagglyOpt(title,"sortOrder") == "desc")
			list = list.reverse();

		var output = [];
		var first = true;
		for (var i=0;i<list.length;i++) {
			if (this.notHidden(list[i],title)) {
				var countString = this.getTaggingCount(list[i].title);
				var excerpt = this.getExcerpt(title,list[i].title);
				if (useCommas)
					output.push((first ? "" : ", ") + "[[" + list[i].title + "]]" + countString + excerpt);
				else
					output.push("*[[" + list[i].title + "]]" + countString + excerpt + "\n");

				first = false;
			}
		}

		return this.drawTable(place,
			this.makeColumns(output,useCommas ? 1 : parseInt(this.getTagglyOpt(title,"numCols"))),
			useCommas ? "commas" : "normal");
	},

	// this is for the "grouped" mode
	createTagglyListGrouped: function(place,title,isTagExpr) {
		var sortBy = this.getTagglyOpt(title,"sortBy");
		var sortOrder = this.getTagglyOpt(title,"sortOrder");

		var list = config.taggly.getTiddlers(title,sortBy,isTagExpr);

		if (sortOrder == "desc")
			list = list.reverse();

		var leftOvers = []
		for (var i=0;i<list.length;i++)
			leftOvers.push(list[i].title);

		var allTagsHolder = {};
		for (var i=0;i<list.length;i++) {
			for (var j=0;j<list[i].tags.length;j++) {

				if (list[i].tags[j] != title) { // not this tiddler

					if (this.notHidden(list[i].tags[j],title)) {

						if (!allTagsHolder[list[i].tags[j]])
							allTagsHolder[list[i].tags[j]] = "";

						if (this.notHidden(list[i],title)) {
							allTagsHolder[list[i].tags[j]] += "**[["+list[i].title+"]]"
										+ this.getTaggingCount(list[i].title) + this.getExcerpt(title,list[i].title) + "\n";

							leftOvers.setItem(list[i].title,-1); // remove from leftovers. at the end it will contain the leftovers

						}
					}
				}
			}
		}

		var allTags = [];
		for (var t in allTagsHolder)
			allTags.push(t);

		var sortHelper = function(a,b) {
			if (a == b) return 0;
			if (a < b) return -1;
			return 1;
		};

		allTags.sort(function(a,b) {
			var tidA = store.getTiddler(a);
			var tidB = store.getTiddler(b);
			if (sortBy == "title") return sortHelper(a,b);
			else if (!tidA && !tidB) return 0;
			else if (!tidA) return -1;
			else if (!tidB) return +1;
			else return sortHelper(tidA[sortBy],tidB[sortBy]);
		});

		var leftOverOutput = "";
		for (var i=0;i<leftOvers.length;i++)
			if (this.notHidden(leftOvers[i],title))
				leftOverOutput += "*[["+leftOvers[i]+"]]" + this.getTaggingCount(leftOvers[i]) + this.getExcerpt(title,leftOvers[i]) + "\n";

		var output = [];

		if (sortOrder == "desc")
			allTags.reverse();
		else if (leftOverOutput != "")
			// leftovers first...
			output.push(leftOverOutput);

		for (var i=0;i<allTags.length;i++)
			if (allTagsHolder[allTags[i]] != "")
				output.push("*[["+allTags[i]+"]]" + this.getTaggingCount(allTags[i]) + this.getExcerpt(title,allTags[i]) + "\n" + allTagsHolder[allTags[i]]);

		if (sortOrder == "desc" && leftOverOutput != "")
			// leftovers last...
			output.push(leftOverOutput);

		return this.drawTable(place,
				this.makeColumns(output,parseInt(this.getTagglyOpt(title,"numCols"))),
				"grouped");

	},

	// used to build site map
	treeTraverse: function(title,depth,sortBy,sortOrder,isTagExpr) {

		var list = config.taggly.getTiddlers(title,sortBy,isTagExpr);

		if (sortOrder == "desc")
			list.reverse();

		var indent = "";
		for (var j=0;j<depth;j++)
			indent += "*"

		var childOutput = "";

		if (depth > this.config.siteMapDepthLimit)
			childOutput += indent + this.lingo.tooDeepMessage;
		else
			for (var i=0;i<list.length;i++)
				if (list[i].title != title)
					if (this.notHidden(list[i].title,this.config.inTiddler))
						childOutput += this.treeTraverse(list[i].title,depth+1,sortBy,sortOrder,false);

		if (depth == 0)
			return childOutput;
		else
			return indent + "[["+title+"]]" + this.getTaggingCount(title) + this.getExcerpt(this.config.inTiddler,title,depth) + "\n" + childOutput;
	},

	// this if for the site map mode
	createTagglyListSiteMap: function(place,title,isTagExpr) {
		this.config.inTiddler = title; // nasty. should pass it in to traverse probably
		var output = this.treeTraverse(title,0,this.getTagglyOpt(title,"sortBy"),this.getTagglyOpt(title,"sortOrder"),isTagExpr);
		return this.drawTable(place,
				this.makeColumns(output.split(/(?=^\*\[)/m),parseInt(this.getTagglyOpt(title,"numCols"))), // regexp magic
				"sitemap"
				);
	},

	macros: {
		tagglyTagging: {
			handler: function (place,macroName,params,wikifier,paramString,tiddler) {
				var parsedParams = paramString.parseParams("tag",null,true);
				var refreshContainer = createTiddlyElement(place,"div");

				// do some refresh magic to make it keep the list fresh - thanks Saq
				refreshContainer.setAttribute("refresh","macro");
				refreshContainer.setAttribute("macroName",macroName);

				var tag = getParam(parsedParams,"tag");
				var expr = getParam(parsedParams,"expr");

				if (expr) {
					refreshContainer.setAttribute("isTagExpr","true");
					refreshContainer.setAttribute("title",expr);
					refreshContainer.setAttribute("showEmpty","true");
				}
				else {
					refreshContainer.setAttribute("isTagExpr","false");
					if (tag) {
        				refreshContainer.setAttribute("title",tag);
						refreshContainer.setAttribute("showEmpty","true");
					}
					else {
        				refreshContainer.setAttribute("title",tiddler.title);
						refreshContainer.setAttribute("showEmpty","false");
					}
				}
				this.refresh(refreshContainer);
			},

			refresh: function(place) {
				var title = place.getAttribute("title");
				var isTagExpr = place.getAttribute("isTagExpr") == "true";
				var showEmpty = place.getAttribute("showEmpty") == "true";
				removeChildren(place);
				addClass(place,"tagglyTagging");
				var countFound = config.taggly.getTiddlers(title,'title',isTagExpr).length
				if (countFound > 0 || showEmpty) {
					var lingo = config.taggly.lingo;
					config.taggly.createListControl(place,title,"hideState");
					if (config.taggly.getTagglyOpt(title,"hideState") == "show") {
						createTiddlyElement(place,"span",null,"tagglyLabel",
								isTagExpr ? lingo.labels.exprLabel.format([title]) : lingo.labels.label.format([title]));
						config.taggly.createListControl(place,title,"title");
						config.taggly.createListControl(place,title,"modified");
						config.taggly.createListControl(place,title,"created");
						config.taggly.createListControl(place,title,"listMode");
						config.taggly.createListControl(place,title,"excerpts");
						config.taggly.createListControl(place,title,"numCols");
						config.taggly.createTagglyList(place,title,isTagExpr);
						if (countFound == 0 && showEmpty)
							createTiddlyElement(place,"div",null,"tagglyNoneFound",lingo.labels.noneFound);
					}
				}
			}
		}
	},

	// todo fix these up a bit
	styles: [
"/*{{{*/",
"/* created by TagglyTaggingPlugin */",
".tagglyTagging { padding-top:0.5em; }",
".tagglyTagging li.listTitle { display:none; }",
".tagglyTagging ul {",
"	margin-top:0px; padding-top:0.5em; padding-left:2em;",
"	margin-bottom:0px; padding-bottom:0px;",
"}",
".tagglyTagging { vertical-align: top; margin:0px; padding:0px; }",
".tagglyTagging table { margin:0px; padding:0px; }",
".tagglyTagging .button { visibility:hidden; margin-left:3px; margin-right:3px; }",
".tagglyTagging .button, .tagglyTagging .hidebutton {",
"	color:[[ColorPalette::TertiaryLight]]; font-size:90%;",
"	border:0px; padding-left:0.3em;padding-right:0.3em;",
"}",
".tagglyTagging .button:hover, .hidebutton:hover, ",
".tagglyTagging .button:active, .hidebutton:active  {",
"	border:0px; background:[[ColorPalette::TertiaryPale]]; color:[[ColorPalette::TertiaryDark]];",
"}",
".selected .tagglyTagging .button { visibility:visible; }",
".tagglyTagging .hidebutton { color:[[ColorPalette::Background]]; }",
".selected .tagglyTagging .hidebutton { color:[[ColorPalette::TertiaryLight]] }",
".tagglyLabel { color:[[ColorPalette::TertiaryMid]]; font-size:90%; }",
".tagglyTagging ul {padding-top:0px; padding-bottom:0.5em; margin-left:1em; }",
".tagglyTagging ul ul {list-style-type:disc; margin-left:-1em;}",
".tagglyTagging ul ul li {margin-left:0.5em; }",
".editLabel { font-size:90%; padding-top:0.5em; }",
".tagglyTagging .commas { padding-left:1.8em; }",
"/* not technically tagglytagging but will put them here anyway */",
".tagglyTagged li.listTitle { display:none; }",
".tagglyTagged li { display: inline; font-size:90%; }",
".tagglyTagged ul { margin:0px; padding:0px; }",
".excerpt { color:[[ColorPalette::TertiaryDark]]; }",
".excerptIndent { margin-left:4em; }",
"div.tagglyTagging table,",
"div.tagglyTagging table tr,",
"td.tagglyTagging",
" {border-style:none!important; }",
".tagglyTagging .contents { border-bottom:2px solid [[ColorPalette::TertiaryPale]]; padding:0 1em 1em 0.5em;",
"  margin-bottom:0.5em; }",
".tagglyTagging .indent1  { margin-left:3em;  }",
".tagglyTagging .indent2  { margin-left:4em;  }",
".tagglyTagging .indent3  { margin-left:5em;  }",
".tagglyTagging .indent4  { margin-left:6em;  }",
".tagglyTagging .indent5  { margin-left:7em;  }",
".tagglyTagging .indent6  { margin-left:8em;  }",
".tagglyTagging .indent7  { margin-left:9em;  }",
".tagglyTagging .indent8  { margin-left:10em; }",
".tagglyTagging .indent9  { margin-left:11em; }",
".tagglyTagging .indent10 { margin-left:12em; }",
".tagglyNoneFound { margin-left:2em; color:[[ColorPalette::TertiaryMid]]; font-size:90%; font-style:italic; }",
"/*}}}*/",
		""].join("\n"),

	init: function() {
		merge(config.macros,this.macros);
		config.shadowTiddlers["TagglyTaggingStyles"] = this.styles;
		store.addNotification("TagglyTaggingStyles",refreshStyles);
	}
};

config.taggly.init();

//}}}

/***
InlineSlidersPlugin
By Saq Imtiaz
http://tw.lewcid.org/sandbox/#InlineSlidersPlugin

// syntax adjusted to not clash with NestedSlidersPlugin
// added + syntax to start open instead of closed

***/
//{{{
config.formatters.unshift( {
	name: "inlinesliders",
	// match: "\\+\\+\\+\\+|\\<slider",
	match: "\\<slider",
	// lookaheadRegExp: /(?:\+\+\+\+|<slider) (.*?)(?:>?)\n((?:.|\n)*?)\n(?:====|<\/slider>)/mg,
	lookaheadRegExp: /(?:<slider)(\+?) (.*?)(?:>)\n((?:.|\n)*?)\n(?:<\/slider>)/mg,
	handler: function(w) {
		this.lookaheadRegExp.lastIndex = w.matchStart;
		var lookaheadMatch = this.lookaheadRegExp.exec(w.source)
		if(lookaheadMatch && lookaheadMatch.index == w.matchStart ) {
			var btn = createTiddlyButton(w.output,lookaheadMatch[2] + " "+"\u00BB",lookaheadMatch[2],this.onClickSlider,"button sliderButton");
			var panel = createTiddlyElement(w.output,"div",null,"sliderPanel");
			panel.style.display = (lookaheadMatch[1] == '+' ? "block" : "none");
			wikify(lookaheadMatch[3],panel);
			w.nextMatch = lookaheadMatch.index + lookaheadMatch[0].length;
		}
   },
   onClickSlider : function(e) {
		if(!e) var e = window.event;
		var n = this.nextSibling;
		n.style.display = (n.style.display=="none") ? "block" : "none";
		return false;
	}
});

//}}}

/***
|Name:|ToggleTagPlugin|
|Description:|Makes a checkbox which toggles a tag in a tiddler|
|Version:|3.1.0 ($Rev: 4907 $)|
|Date:|$Date: 2008-05-13 03:15:46 +1000 (Tue, 13 May 2008) $|
|Source:|http://mptw.tiddlyspot.com/#ToggleTagPlugin|
|Author:|Simon Baird <simon.baird@gmail.com>|
|License:|http://mptw.tiddlyspot.com/#TheBSDLicense|
!!Usage
{{{<<toggleTag }}}//{{{TagName TiddlerName LabelText}}}//{{{>>}}}
* TagName - the tag to be toggled, default value "checked"
* TiddlerName - the tiddler to toggle the tag in, default value the current tiddler
* LabelText - the text (gets wikified) to put next to the check box, default value is '{{{[[TagName]]}}}' or '{{{[[TagName]] [[TiddlerName]]}}}'
(If a parameter is '.' then the default will be used)
* TouchMod flag - if non empty then touch the tiddlers mod date. Note, can set config.toggleTagAlwaysTouchModDate to always touch mod date
!!Examples
|Code|Description|Example|h
|{{{<<toggleTag>>}}}|Toggles the default tag (checked) in this tiddler|<<toggleTag>>|
|{{{<<toggleTag TagName>>}}}|Toggles the TagName tag in this tiddler|<<toggleTag TagName>>|
|{{{<<toggleTag TagName TiddlerName>>}}}|Toggles the TagName tag in the TiddlerName tiddler|<<toggleTag TagName TiddlerName>>|
|{{{<<toggleTag TagName TiddlerName 'click me'>>}}}|Same but with custom label|<<toggleTag TagName TiddlerName 'click me'>>|
|{{{<<toggleTag . . 'click me'>>}}}|dot means use default value|<<toggleTag . . 'click me'>>|
!!Notes
* If TiddlerName doesn't exist it will be silently created
* Set label to '-' to specify no label
* See also http://mgtd-alpha.tiddlyspot.com/#ToggleTag2
!!Known issues
* Doesn't smoothly handle the case where you toggle a tag in a tiddler that is current open for editing
* Should convert to use named params
***/
//{{{

if (config.toggleTagAlwaysTouchModDate == undefined) config.toggleTagAlwaysTouchModDate = false;

merge(config.macros,{

	toggleTag: {

		createIfRequired: true,
		shortLabel: "[[%0]]",
		longLabel: "[[%0]] [[%1]]",

		handler: function(place,macroName,params,wikifier,paramString,tiddler) {
			var tiddlerTitle = tiddler ? tiddler.title : '';
			var tag   = (params[0] && params[0] != '.') ? params[0] : "checked";
			var title = (params[1] && params[1] != '.') ? params[1] : tiddlerTitle;
			var defaultLabel = (title == tiddlerTitle ? this.shortLabel : this.longLabel);
			var label = (params[2] && params[2] != '.') ? params[2] : defaultLabel;
			var touchMod = (params[3] && params[3] != '.') ? params[3] : "";
			label = (label == '-' ? '' : label); // dash means no label
			var theTiddler = (title == tiddlerTitle ? tiddler : store.getTiddler(title));
			var cb = createTiddlyCheckbox(place, label.format([tag,title]), theTiddler && theTiddler.isTagged(tag), function(e) {
				if (!store.tiddlerExists(title)) {
					if (config.macros.toggleTag.createIfRequired) {
						var content = store.getTiddlerText(title); // just in case it's a shadow
						store.saveTiddler(title,title,content?content:"",config.options.txtUserName,new Date(),null);
					}
					else 
						return false;
				}
				if ((touchMod != "" || config.toggleTagAlwaysTouchModDate) && theTiddler)
						theTiddler.modified = new Date();
				store.setTiddlerTag(title,this.checked,tag);
				return true;
			});
		}
	}
});

//}}}

!Description
Extracts all hook metrics from a [[proboscisProfiles]] object.

!Usage
//{{{
allHookMetrics(proboscisProfiles)
//}}}

!Arguments
{{{proboscisProfiles}}} - an object of class [[proboscisProfiles]]

!Value
A matrix where rows are specimens and columns are moving average centres (%) for each metric: l (length) and b (base).
!Description
Extracts annotation from a [[proboscisProfiles]] object.

!Usage
//{{{
annotation(proboscisProfiles)
//}}}

!Arguments
{{{proboscisProfiles}}} - an object of class [[proboscisProfiles]]

!Value
A data.frame with three columns: specimen, group and numberHooks.
!Description
Extracts hook base profiles from a proboscisProfiles object.

!Usage
//{{{
hookBaseProfiles(proboscisProfiles)
//}}}

!Arguments
{{{proboscisProfiles}}} - an object of class proboscisProfiles

!Value
A matrix of hook base widths where rows are specimens and columns are moving average centres (%).
!Description
Function for extracting hook data from [[hookMeasurements]] object.

!Usage
//{{{
hookData(object)
//}}}

!Arguments
{{{object}}} - an object of class [[hookMeasurements]]

!Value
A data.frame with the following eight columns: specimen, group, hook, stdPos, length, base, area and ratio.
!Description
Extracts hook length profiles from a proboscisProfiles object.

!Usage
//{{{
hookLengthProfiles(proboscisProfiles)
//}}}

!Arguments
{{{proboscisProfiles}}} - an object of class proboscisProfiles

!Value
A matrix of hook lengths where rows are specimens and columns are moving average centres (%).
!Description
Class for raw hook measurement data

!Definition
//{{{
setClass("hookMeasurements", representation(hookData = "data.frame", notes="character"))

validHookMeasurements <- function(object)
	{
		if(names(object@hookData)[1] != "specimen")
			return(cat("First column in hookData data.frame must be specimen\n"))
		else if(names(object@hookData)[2] != "group")
			return(cat("Second column in hookData data.frame must be group\n"))
		else if(names(object@hookData)[3] != "hook")
			return(cat("Third column in hookData data.frame must be hook\n"))
		else if(names(object@hookData)[4] != "stdPos")
			return(cat("Fourth column in hookData data.frame must be stdPos\n"))
		else if(names(object@hookData)[5] != "length")
			return(cat("Fifth column in hookData data.frame must be length\n"))
		else if(names(object@hookData)[6] != "base")
			return(cat("Sixth column in hookData data.frame must be base\n"))
		else if(length(object@hookData[1,]) != 6)
			return(cat("You must supply a data.frame with exactly six columns: specimen, group, hook, stdPos, length, base.\n"))
		else if(is.element(TRUE, unlist(is.na(object@hookData))))
			return(cat("Supplied data.frame contains missing data\n"))
		else if(!is.integer(object@hookData$hook))
			return(cat("Hook position must be an integer\n"))
		else if(!is.numeric(object@hookData$stdPos))
			return(cat("Hook standardized position must be numeric\n"))
		else if(!is.numeric(object@hookData$length))
			return(cat("Hook length measurement must be numeric\n"))
		else if(!is.numeric(object@hookData$base))
			return(cat("Hook base measurement must be numeric\n"))
	else return(TRUE)
	}

setValidity("hookMeasurements", validHookMeasurements)

# predicate function
is.hookMeasurements <- function(x) inherits(x, "hookMeasurements")
//}}}

!Methods
[[summary.hookMeasurements]]
[[hooksPerRow]]
[[hookData]]
[[writeHookMeasurements]]
[[profileProboscides]]
[[minMovAvgSeg]]
!Description
Class for summaries of objects of class [[hookMeasurements]]

!Definition
//{{{
setClass("hookMeasurementsSummary", representation(numGroups="integer", numSpecimens="integer", detail="data.frame", notes="character"))

validHookMeasurementsSummary <- function(object)
	{
		if(length(object@numGroups) < 1)
			return("There must be at least one group")
		else if(length(object@numSpecimens) < 1)
			return("There must be at least one specimen")
		else if(length(object@detail[1,]) != 2)
			return("The detail data.frame must contain exactly two columns: group, number of specimens")
		else return(TRUE)
	}
	
setValidity("hookMeasurementsSummary", validHookMeasurementsSummary)

#predicate function
is.hookMeasurementsSummary <- function(x) inherits(x, "hookMeasurementsSummary")

//}}}

!Methods
[[print.hookMeasurementsSummary]]
[[show.hookMeasurementsSummary]]
!Description
Function for returning the number of hooks per longitudinal row for each specimen in a [[hookMeasurements]] object.

!Usage
//{{{
hooksPerRow(object)
//}}}

!Arguments
{{{object}}} - an object of class [[hookMeasurements]]

!Value
A data.frame with three columns: specimen, group and numberHooks
!Description
Function for calculating the minimum moving average interval that can be applied to a [[hookMeasurements]] object using the [[profileProboscides]] function.

!Usage
//{{{
minMovAvgSeg(hookMeasurements)
//}}}

!Arguments
{{{hookMeasurements}}} - an object of class [[hookMeasurements]]

!Value
An object of class numeric
!Description
Yields moving average interval used in generation of proboscis profiles.

!Usage
//{{{
movAvgSeg(proboscisProfiles)
//}}}

!Arguments
{{{proboscisProfiles}}} - an object of class [[proboscisProfiles]]

!Value
An object of class numeric.
!Description
Function for creating an object of class [[hookMeasurements]]

!Usage
//{{{
newHookMeasurements(rawData, notes="")
//}}}

!Arguments
{{{rawData}}} - a data.frame containing the raw hook data
{{{notes}}} - optional character string of notes

!Value
Returns an object of class [[hookMeasurements]]
!Description
Extracts notes (if they exist) from a hookMeasurements or proboscisProfiles object.

!Usage
notes(x)

!Arguments
{{{x}}} - an object of class [[hookMeasurements]] or [[proboscisProfiles]]

!Value
An object of class character
!Description
Function to print objects of class [[hookMeasurementsSummary]]

!Usage
//{{{
print(object)
//}}}
or
//{{{
print.hookMeasurementsSummary(object)
//}}}

!Arguments
{{{object}}} - an object of class [[hookMeasurementsSummary]]

!Description
Function to print objects of class [[proboscisProfilesSummary]]

!Usage
//{{{
print(object)
//}}}
or
//{{{
print.proboscisProfilesSummary(object)
//}}}

!Arguments
{{{object}}} - an object of class [[proboscisProfilesSummary]]

!Description
Class for proboscis profiles.

!Definition
//{{{
setClass("proboscisProfiles", representation(movAvgSeg="numeric", Length="matrix", Base="matrix", annotation="data.frame", notes="character"))

is.proboscisProfiles <- function(x) inherits(x, "proboscisProfiles")

//}}}

!Methods
[[summary.proboscisProfiles]]
[[hookLengthProfiles]]
[[writeHookLengthProfiles]]
[[hookBaseProfiles]]
[[writeHookBaseProfiles]]
[[allHookMetrics]]
[[writeAllHookMetrics]]
[[annotation]]
[[movAvgSeg]]
[[notes]]
[[proboscisProfilesDataFrame]]

!Description
Puts data in a [[proboscisProfiles]] object into a data.frame, which is an easier format for plotting profiles, etc.

!Usage
//{{{
proboscisProfilesDataFrame(x)
//}}}

!Arguments
{{{x}}} - an object of class [[proboscisProfiles]]

!Value
An object of class data.frame with the following columns: "specimen", "group", "position", "length" and "base".
!Description
Class for summaries of objects of class [[proboscisProfiles]]

!Definition
//{{{
setClass("proboscisProfilesSummary", representation(movAvgSeg="numeric", numGroups="integer", numSpecimens="integer", detail="data.frame", notes="character"))

validProboscisProfilesSummary <- function(object)
	{
		if(length(object@numGroups) < 1)
			return(cat("There must be at least one group\n"))
		else if(length(object@numSpecimens) < 1)
			return(cat("There must be at least one specimen\n"))
		else if(length(object@detail[1,]) != 2)
			return(cat("The detail data.frame must contain exactly two columns: group, number of specimens\n"))
		else return(TRUE)
	}
	
setValidity("proboscisProfilesSummary", validProboscisProfilesSummary)

is.ProboscisProfilesSummary <- function(x) inherits(x, "proboscisProfilesSummary")
//}}}


!Methods
[[print.proboscisProfilesSummary]]
[[show.proboscisProfilesSummary]]
!Description
Function for generating proboscis profiles from data stored in a [[hookMeasurements]] object.

!Usage
//{{{
profileProboscides(hookMeasurements, movAvgSeg, ln)
//}}}

!Arguments
{{{hookMeasurements}}} - an object of class [[hookMeasurements]]
{{{movAvgSeg}}} - optional numeric value for size of moving average interval (defaults to minimum moving average interval that can be applied to the dataset).
{{{ln}}} - a logical value (TRUE or FALSE) to indicate if output hook metrics should be transformed to natural logarithms (default is TRUE).

!Value
An object of class [[proboscisProfiles]]
!Description
Function for reading in a file of raw hook data and creating an object of class [[hookMeasurements]]. A wrapper to [[newHookMeasurements]]

!Usage
//{{{
readHookMeasurements(filename, notes='')
//}}}

!Arguments
{{{filename}}} - path to file containing raw hook data
{{{notes}}} - optional character string of notes

!Value
Returns an object of class [[hookMeasurements]]
!Description
Function to display objects of class [[hookMeasurementsSummary]]. Equivalent to [[print.hookMeasurementsSummary]]. The show function is used for automatic printing of objects.

!Usage
//{{{
show(object)
//}}}
or
//{{{
show.hookMeasurementsSummary(object)
//}}}

!Arguments
{{{object}}} - an object of class [[hookMeasurementsSummary]]
!Description
Function to display objects of class [[proboscisProfilesSummary]]. Equivalent to [[print.proboscisProfilesSummary]]. The show function is used for automatic printing of objects.

!Usage
//{{{
show(object)
//}}}
or
//{{{
show.proboscisProfilesSummary(object)
//}}}

!Arguments
{{{object}}} - an object of class [[proboscisProfilesSummary]]
!Description
Summary method for objects of class [[hookMeasurements]]

!Usage
//{{{
summary(object)
//}}}

or
//{{{
summary.hookMeasurements(object)
//}}}

!Arguments
{{{object}}} - an object of class [[hookMeasurements]]

!Value
Returns an object of class [[hookMeasurementsSummary]]
!Description
Summary method for objects of class [[proboscisProfiles]]

!Usage
//{{{
summary(object)
//}}}

or
//{{{
summary.proboscisProfiles(object)
//}}}


!Arguments
{{{object}}} - an object of class [[proboscisProfiles]]

!Value
Returns an object of class [[proboscisProfilesSummary]]

!Description
Writes profile data for all hook metrics to file.

!Usage
//{{{
writeAllHookMetrics(proboscisProfiles, filename)
//}}}

!Arguments
{{{proboscisProfiles}}} - an object of class [[proboscisProfiles]]
{{{filename}}} - character string naming a file
!Description
Extracts hook base profiles from a [[proboscisProfiles]] object and writes them to file.

!Usage
//{{{
writeHookBaseProfiles(proboscisProfiles, filename)
//}}}

!Arguments
{{{proboscisProfiles}}} - an object of class [[proboscisProfiles]]
{{{filename}}} - character string naming a file
!Description
Extracts hook length profiles from a [[proboscisProfiles]] object and writes them to file.

!Usage
writeHookLengthProfiles(proboscisProfiles, filename)

!Arguments
{{{proboscisProfiles}}} - an object of class [[proboscisProfiles]]
{{{filename}}} - character string naming a file
!Description
Function to write hook data from a [[hookMeasurements]] object to a csv text file. File will contain the following six columns: specimen, group, hook, stdPos, length and base.

!Usage
//{{{
writeHookMeasurements(hookMeasurements, filename)
//}}}

!Arguments
{{{hookMeasurements}}} - an object of class hookMeasurements
{{{filename}}} - character string naming a file